Alexa Fluor® 700 anti-human CD14 Antibody
- 产品名称：
- Alexa Fluor® 700 anti-human CD14 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 301822
- 产品应用：
- 301822
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Capuchin Monkey, Cow, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Dog, Pigtailed Macaque, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length human CD14 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 301861 and 301862).
- Application References
(PubMed link indicates BioLegend citation) -
- McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
- Williams KC, et al. 2001. J. Exp. Med. 193:905.
- Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
- Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- Dudley D, et al. 2016. Nat Commun. 7:12204. PubMed
- Li H, et al. 2015. J Infect Dis. 211:1717. PubMed
- Kim ST, et al. 2022. Nat Commun. 13:1970. PubMed
- Verma A, et al. 2021. Cell Rep. 37:109942. PubMed
- Judge CJ, et al. 2017. J Leukoc Biol. 102:171. PubMed
- Boyle M, et al. 2015. J Infect Dis. 212: 416-425. PubMed
- Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
- Jagannathan P, et al. 2017. Sci Rep. 10.1038/s41598-017-10624-3. PubMed
- Lewis SA, et al. 2021. Front Immunol. 12:724015. PubMed
- Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
- Sureshchandra S, et al. 2021. iScience. 24:102690. PubMed
- Sabree SA, et al. 2021. J Immunother Cancer. 9: . PubMed
- Sureshchandra S, et al. 2022. Cell Rep. 39:110938. PubMed
- Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
- Pham TNQ, et al. 2020. Cell Reports. 29(9):2770-2782.e5.. PubMed
- Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
- Pi J, et al. 2022. J Nanobiotechnology. 20:36. PubMed
- Castellano E, et al. 2022. Front Immunol. 13:970931. PubMed
- O'Connor MH, et al. 2021. Commun Biol. 4:563. PubMed
- Clayton KL, et al. 2021. Cell Host Microbe. 29(3):435-447.e9. PubMed
- Connors J, et al. 2022. Geroscience. :. PubMed
- RRID
- AB_493747 (BioLegend Cat. No. 301822)
- Structure
- GPI-linked membrane glycoprotein, 53-55 kD
- Distribution
-
Monocytes, macrophages, granulocytes (low)
- Function
- LPS receptor, clearance of Gram-negative pathogens
- Ligand/Receptor
- LPS
- Cell Type
- Granulocytes, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.
- Gene ID
- 929 View all products for this Gene ID
- UniProt
- View information about CD14 on UniProt.org
