This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-A^b, I-A^d, I-A^q, I-E^d, and I-E^k MHC class II alloantigens from mice carrying H-2^p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-A^f, I-A^k, or I-A^s MHC class II alloantigens.¹

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemistry of frozen sections^2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding^4-7, and spatial biology (IBEX)^17,18. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Maxpar? is a registered trademark of Standard BioTools Inc.

1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
2. Viville S, et al. 1993. Cell 72:635. (IHC)
3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
6. Guo M, et al. 1995. Zygote 3:65. (IHC)
7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
17. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
18. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

1. Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
2. Dasgupta D, et al. 2020. Gastroenterology. 159:1487. PubMed
3. Wei SC, et al. 2019. Immunity. 50:1084. PubMed
4. Barvalia M, et al. 2022. Methods Mol Biol. 2508:147. PubMed
5. Han G, et al. 2018. Nat Protoc. 2.014583333. PubMed
6. Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed

B cell and activated T cells, APCs of the H-2^b,d,q,r bearing mice

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.