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Brilliant Violet 650™ anti-mouse I-A/I-E Ant
产品名称：
Brilliant Violet 650™ anti-mouse I-A/I-E Ant
产品类别：
抗体
产品编号：
107641
产品应用：
107641
[价格]
规格 价格 库存
50µg ￥ 3276 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650? under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650? excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  18. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  2. Giorgetti E, et al. 2020. Cell Reports. 29(6):1539-1554.e7.. PubMed
  3. Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
  4. Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed
  5. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  6. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  7. van der Lelie D, et al. 2021. Nat Commun. 12:3105. PubMed
  8. Tuttle KD, et al. 2020. Cell Rep. 33:108407. PubMed
  9. Darragh LB, et al. 2022. Nat Commun. 13:7015. PubMed
  10. Stutz MD, et al. 2021. Immunity. 54(8):1758-1771.e7. PubMed
  11. Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed
  12. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  13. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  14. Ben-Yehuda H, et al. 2021. Mol Neurodegener. 16:39. PubMed
  15. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  16. Molgora M, et al. 2020. Cell. 182:886. PubMed
  17. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  18. Schuh E, et al. 2017. J Immunol. 198:3081. PubMed
  19. Goc J, et al. 2021. Cell. :. PubMed
  20. Fearon AE, et al. 2021. iScience. 24:103143. PubMed
  21. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
RRID
AB_2565975 (BioLegend Cat. No. 107641)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

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