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首页 > 抗体蛋白 > 抗体
Brilliant Violet 510™ anti-mouse I-A/I-E Ant
产品名称：
Brilliant Violet 510™ anti-mouse I-A/I-E Ant
产品类别：
抗体
产品编号：
107635
产品应用：
107635
[价格]
规格 价格 库存
125µL ￥ 2112 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
?g & ?l: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
?g & ?l: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510? under optimal conditions.
Concentration
?g size: 0.2 mg/mL
?L size: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
?g & ?l: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using ?g size, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. For flow cytometric staining using ?l size, the suggested use of this reagent is 5 ?l per million cells or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510? excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  18. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Gogoi H, et al. 2020. Front Immunol. 1.620833333. PubMed
  2. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  3. Wenzek C, et al. 2022. iScience. 25:105540. PubMed
  4. Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed
  5. Ogbechi J, et al. 2022. Front Immunol. 13:1001956. PubMed
  6. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
  7. Kashyap AS, et al. 2019. Cell Rep. 28:3367. PubMed
  8. Diao L, et al. 2022. iScience. 25:105511. PubMed
  9. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  10. Yousif AS, et al. 2020. Immunity. 54(2):235-246.e5. PubMed
  11. Chikina AS, et al. 2020. Cell. 183:411. PubMed
  12. Zhu X, et al. 2017. Arch Oral Biol. 10.1016/j.archoralbio.2017.03.010. PubMed
  13. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  14. Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
  15. Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed
  16. Esmaili S, et al. 2021. Cell Systems. 12(5):432-445.e7. PubMed
  17. Eddy W, et al. 2017. The Journal of Immunology. 10.4049/jimmunol.1601777. PubMed
  18. Moreau HD et al. 2019. Dev Cell. 49(2):171-188 . PubMed
  19. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  20. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  21. Wu X, et al. 2021. Elife. 10:. PubMed
  22. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  23. Kuhn NF, et al. 2020. Nat Commun. 4.74375. PubMed
  24. Huggins MA, et al. 2019. Cell Rep. 28:1729. PubMed
  25. Stump CT, et al. 2021. Open Biol. 11:210245. PubMed
  26. Sato R, et al. 2020. Int Immunol. 499:32. PubMed
  27. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  28. Kelsey E Sivick et al. 2018. Cell reports. 25(11):3074-3085 . PubMed
  29. Garcia LR, et al. 2021. Nat Commun. 12:3364. PubMed
  30. Huang YL, et al. 2021. Commun Biol. 4:229. PubMed
  31. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  32. Formaglio P, et al. 2021. Immunity. 54:2724. PubMed
  33. Baasch S, et al. 2021. Cell. . PubMed
  34. Lim HY et al. 2018. Immunity. 49(2):326-341 . PubMed
  35. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  36. Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed
  37. Sangesland M, et al. 2020. Cell Reports. 32(8):108065. PubMed
  38. Chauveau L, et al. 2021. EMBO Rep. 22:e52447. PubMed
  39. Scur M, et al. 2022. Nat Commun. 13:7272. PubMed
  40. Monaghan KL, et al. 2020. J Vis Exp. . PubMed
  41. Lammers K, et al. 2015. PLoS One. 10: 0138338. PubMed
  42. Ermert D, et al. 2016. PLoS Pathog. 11: 1005043. PubMed
  43. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  44. Liu S, et al. 2018. Front Immunol. 29:208. PubMed
  45. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  46. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  47. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  48. Li E, et al. 2021. Front Immunol. 12:667177. PubMed
  49. Yasuda T, et al. 2016. PLoS One. 11:e0167952. PubMed
  50. Hammer A, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01922. PubMed
  51. Hollern DP, et al. 2020. Cell. 179(5):1191-1206.e21.. PubMed
  52. Rosato PC, et al. 2019. Nat Commun. 10:567. PubMed
  53. Katzmarski N, et al. 2021. Nat Immunol. 22:1382. PubMed
  54. Truckenbrod EN, et al. 2021. Elife. 10:. PubMed
  55. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  56. Knuplez E, et al. 2021. British Journal of Pharmacology. 178(5):1234-1248. PubMed
  57. Velazquez VM, et al. 2017. Mol Ther Methods Clin Dev. 0.277083333. PubMed
  58. Hartwig T et al. 2017. Molecular cell. 65(4):730-742 . PubMed
  59. Walker JA, et al. 2020. Immunity. 51(1):104-118. PubMed
  60. Zhang J, et al. 2020. Cell Metabolism. 31(6):1136-1153. PubMed
  61. Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
  62. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  63. Kuhn NF et al. 2019. Cancer cell. 35(3):473-488 . PubMed
RRID
AB_2561397 (BioLegend Cat. No. 107635) AB_2734168 (BioLegend Cat. No. 107636)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

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