Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-A^b, I-A^d, I-A^q, I-E^d, and I-E^k MHC class II alloantigens from mice carrying H-2^p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-A^f, I-A^k, or I-A^s MHC class II alloantigens.¹

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemistry of frozen sections^2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding^4-7, and spatial biology (IBEX)^17,18. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

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4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
6. Guo M, et al. 1995. Zygote 3:65. (IHC)
7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
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11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
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14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
17. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
18. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

1. Boyd DF, et al. 2020. Nature. 587:466. PubMed
2. Matson CA, et al. 2020. Proc Natl Acad Sci U S A. 117:14342. PubMed
3. Van de Velde L, et al. 2016. Cell Rep. 17:2247-2258. PubMed
4. Gajdasik DW, et al. 2020. Nat Commun. 2.834027778. PubMed
5. Bonnardel J, et al. 2019. Immunity. 51:638. PubMed
6. Kaplan D, et al. 2007. J Exp Med. 204:2545. PubMed
7. Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed
8. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
9. Akkaya B, et al. 2017. J Immunol Methods. 441:67. PubMed
10. Tian Q, et al. 2021. Infect Immun. 89:e0020521. PubMed
11. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed

B cell and activated T cells, APCs of the H-2^b,d,q,r bearing mice

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.