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首页 > 抗体蛋白 > 抗体
APC anti-mouse I-A/I-E Antibody
产品名称：
APC anti-mouse I-A/I-E Antibody
产品类别：
抗体
产品编号：
107613
产品应用：
107613
[价格]
规格 价格 库存
25µg ￥ 792 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining,the suggested use of this reagent is ≤ 0.25 ?g per 106cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  18. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Combes F, et al. 2018. Neoplasia. 20:848. PubMed
  2. Ying W, et al. 2019. Cell Metab. 29:457. PubMed
  3. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  4. Kitur K, et al. 2016. Cell Rep. 16:2219-2230. PubMed
  5. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  6. Amir M, et al. 2020. Biochem Biophys Res Commun. 527:1000. PubMed
  7. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  8. Shu T, et al. 2022. Eur Respir J. Online ahead of print. PubMed
  9. Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed
  10. Ma C, et al. 2021. Signal Transduct Target Ther. 6:353. PubMed
  11. Saito S, et al. 2020. Nutrients. 12:. PubMed
  12. Liu X, et al. 2020. Nature. . PubMed
  13. Rothchild AC, et al. 2020. Bio Protoc. 10:00. PubMed
  14. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  15. Mitsune A, et al. 2021. Respir Res. 22:232. PubMed
  16. Lee JH, et al. 2022. iScience. 25:104166. PubMed
  17. Yeung AK, et al. 2020. Blood Adv. 4:6204. PubMed
  18. Teng ZX, et al. 2022. J Inflamm Res. 15:3187. PubMed
  19. Park S, et al. 2021. Nat Cell Biol. 23:476. PubMed
  20. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  21. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  22. Lee M, et al. 2014. PLoS One. 9:112666. PubMed
  23. Müller A, et al. 2020. J Clin Invest. 5765:130. PubMed
  24. Kohn M, et al. 2021. Front Immunol. 580594:12. PubMed
  25. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  26. Rayamajhi M, et al. 2010. J Exp Med. 207:327. PubMed
  27. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  28. Gray D, et al. 2007. J Exp Med. 204:2521. PubMed
  29. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  30. Al Mamun A, et al. 2019. Neurochem Int. 127:148. PubMed
  31. Si Y, et al. 2018. J Control Release. 282:120. PubMed
  32. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  33. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  34. Xu X, et al. 2018. Cell. 175:1336. PubMed
  35. Jorapur A, et al. 2022. PLoS Pathog. 18:e1010200. PubMed
  36. Juncheng P, et al. 2022. J Immunother Cancer. 10:. PubMed
  37. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
  38. Van Winkle JA et al. 2018. Cell host & microbe. 24(5):665-676 . PubMed
  39. Levesque S, et al. 2019. Oncoimmunology. 8:e1657375. PubMed
  40. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  41. Milner MT, et al. 2022. STAR Protoc. 3:101295. PubMed
  42. Han F, et al. 2020. Invest Ophthalmol Vis Sci. 61:24. PubMed
  43. Unudurthi SD, et al. 2020. Life Sci. 117440:247. PubMed
  44. Olson GS, et al. 2021. Cell Reports. 35(9):109195. PubMed
  45. Boucard-Jourdin M, et al. 2016. J Immunol. 197: 1968 - 1978. PubMed
  46. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  47. Zhou J, et al. 2019. Nat Commun. 10:2427. PubMed
  48. Taylor D, et al. 2022. Front Immunol. 13:936129. PubMed
  49. Mattila JT, et al. 2017. J Immunol. 199:806. PubMed
  50. Zhu W, et al. 2019. FASEB J. 33:5208. PubMed
  51. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  52. Morikawa M, et al. 2016. PLoS One. 11:e0163607. PubMed
  53. Kitur K, et al. 2015. PLoS Pathog. 11:1004820. PubMed
  54. Wedekind MF, et al. 2021. iScience. 24(7):102759. PubMed
  55. Liu P, et al. 2020. Mol Cell. 748:77. PubMed
  56. Zhao H, et al. 2022. Commun Biol. 5:74. PubMed
  57. Meunier I, et al. 2015. PLoS One. 10: 0138055. PubMed
  58. Liu Y, et al. 2019. Sci Rep. 9:18970. PubMed
  59. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  60. Fitzpatrick Z, et al. 2020. Nature. 587:472. PubMed
  61. Tassi I, et al. 2014. J Immunol. 193:4303. PubMed
  62. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  63. Dheilly E, et al. 2020. Cancer Cell. 37(5):674-689. PubMed
  64. Zhu Y, et al. 2021. Int J Oral Sci. 13:25. PubMed
  65. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  66. Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed
  67. Goc J, et al. 2021. Cell. :. PubMed
  68. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  69. Mangal JL, et al. 2020. J Mater Chem B. 8:5195. PubMed
  70. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  71. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
  72. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  73. Omenetti S, et al. 2019. Immunity. 51:77. PubMed
  74. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
RRID
AB_313328 (BioLegend Cat. No. 107613) AB_313329 (BioLegend Cat. No. 107614)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

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