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Alexa Fluor® 647 anti-mouse I-A/I-E Antibody
产品名称：
Alexa Fluor® 647 anti-mouse I-A/I-E Antibody
产品类别：
抗体
产品编号：
107617
产品应用：
107617
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  18. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Shen Y, et al. 2021. Comput Struct Biotechnol J. 19:5360. PubMed
  2. Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed
  3. Snyder LM, et al. 2021. PLoS Pathog. 17:e1009970. PubMed
  4. Mercer HL, et al. 2020. PLoS Pathog. 16:e1008572. PubMed
  5. Zhang Y, et al. 2015. PLoS Pathog. 11: e1005167. PubMed
  6. Alves C, et al. 2015. PLoS One. 10: 0142972. PubMed
  7. Guo P, et al. 2021. J Immunol. 207:408. PubMed
  8. Peralta Ramos JM, et al. 2017. Front Immunol. 1.490277778. PubMed
  9. Wang ECE, et al. 2019. Cell Stem Cell. 24:654. PubMed
  10. Benson S, Ernst J 2009. PLoS One. 4:e6329. PubMed
  11. Becker W, et al. 2021. J Crohns Colitis. 15:1032. PubMed
  12. Dawson CA, et al. 2021. Nature Protocols. 16(4):1907-1935. PubMed
  13. Uderhardt S, et al. 2019. Cell. 177:541. PubMed
  14. Zhang J, et al. 2021. Nature. 590:457. PubMed
  15. Martinez-Turtos A, et al. 2022. Oncoimmunology. 11:2116844. PubMed
  16. Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
  17. Prizant H, et al. 2021. Cell Reports. 36(6):109523. PubMed
  18. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  19. Hamaidi I, et al. 2020. Cell Metabolism. 32(3):420-436.e12. PubMed
  20. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  21. Boross P, et al. 2014. J Immunol. 193:5506. PubMed
  22. Sancho-Pelluz J, et al. 2008. Invest Ophthalmol Vis Sci. 49:5602. PubMed
  23. Grayczyk JP et al. 2017. Cell host & microbe. 22(5):678-687 . PubMed
  24. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  25. Nicolai L, et al. 2021. Haematologica. Online ahead of print. PubMed
  26. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  27. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  28. Markey KA et al. 2019. Current protocols in immunology. 125(1):e72 . PubMed
RRID
AB_493526 (BioLegend Cat. No. 107617) AB_493525 (BioLegend Cat. No. 107618)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

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