Alexa Fluor® 594 anti-mouse I-A/I-E Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3036	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Activated C57BL/6 mouse spleen cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

IHC-F - Quality tested
3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 0.1 - 10 μg/ml is recommended.?For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-A^b, I-A^d, I-A^q, I-E^d, and I-E^k MHC class II alloantigens from mice carrying H-2^p,r,q,b,d,uhaplotypes. Clone M5/114.15.2 however does not react wtih I-A^f, I-A^k, or I-A^s MHC class II alloantigens.¹

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemistry of frozen sections^2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding^4-7, and spatial biology (IBEX)^17,18. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References (PubMed link indicates BioLegend citation)

Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
Viville S, et al. 1993. Cell 72:635. (IHC)
Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
Yamashita I, et al. 1993. Int. Immunol. 5:1139.
Guo M, et al. 1995. Zygote 3:65. (IHC)
Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
Venanzi ES, et al. 2007. J. Immunol. 179:5693.
Christensen SR, et al. 2006. Immunity 25:417. PubMed
Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
Kuns RD, et al. 2009. Blood 113:5999. PubMed
Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Dixit S, et al. 2018. Biomaterials. 159:130. PubMed

RRID

AB_2566438 (BioLegend Cat. No. 107650)

Antigen Details

Structure: MHC class II
Distribution: B cell and activated T cells, APCs of the H-2^b,d,q,r bearing mice
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD4
Cell Type: Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
Gene ID: 14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt: View information about I-A/I-E on UniProt.org

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