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首页 > 抗体蛋白 > 抗体
Alexa Fluor® 488 anti-mouse I-A/I-E Antibody
产品名称：
Alexa Fluor® 488 anti-mouse I-A/I-E Antibody
产品类别：
抗体
产品编号：
107615
产品应用：
107615
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  18. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed
  2. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  3. Haratani K, et al. 2019. J Clin Invest. 130:374. PubMed
  4. Michelet X, et al. 2015. J Immunol. 194:2079. PubMed
  5. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  6. Zhang K, et al. 2018. PLoS Pathog. 14:e1006925. PubMed
  7. Guzmán M, et al. 2020. Immunology. 161:148. PubMed
  8. Zhang S, et al. 2021. Cell. 184(8):2151-2166.e16. PubMed
  9. Sakai M, et al. 2020. Immunity. 51(4):655-670. PubMed
  10. Tian D, et al. 2020. FASEB J. 34:3367. PubMed
  11. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  12. Park S, et al. 2021. Nat Cell Biol. 23:476. PubMed
  13. Angelidis I, et al. 2019. Nat Commun. 10:963. PubMed
  14. Xu D et al. 2018. Cell. 174(6):1477-1491 . PubMed
  15. Olgu?n-Alor R, et al. 2016. PLoS One. 11:e0167813. PubMed
  16. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  17. Hossain DMS, et al. 2018. J Clin Invest. 128:644. PubMed
  18. Yang C, et al. 2021. J Invest Dermatol. 810:141. PubMed
  19. Aktories P, et al. 2022. Cell Rep Methods. 2:100260. PubMed
  20. Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed
  21. Chung L et al. 2018. Cell host & microbe. 23(2):203-214 . PubMed
  22. Anderson AE, et al. 2022. NPJ Regen Med. 7:6. PubMed
  23. Sun L, et al. 2015. Sci Rep. 5: 14871. PubMed
  24. Napp J, et al. 2019. Theranostics. 4.754861111. PubMed
  25. Hofmann J, et al. 2021. Front Immunol. 11:599495. PubMed
  26. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  27. Liu Z, et al. 2020. EMBO J. 39:e104862. PubMed
  28. Yang H, et al. 2020. Arterioscler Thromb Vasc Biol. 40:e350. PubMed
  29. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  30. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  31. Muchenditsi A, et al. 2017. Am J Physiol Gastrointest Liver Physiol. 313:G39. PubMed
  32. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
  33. Khoury MK, et al. 2021. JVS Vasc Sci. 1:123. PubMed
  34. Reed-Geaghan EG, et al. 2020. J Exp Med. 217:00:00. PubMed
  35. Korin B, et al. 2020. Sleep. :43. PubMed
  36. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  37. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  38. O'Reilly LA, et al. 2018. Immunity. 48:570. PubMed
  39. Lynn GM, et al. 2020. Nat Biotechnol. 38:320. PubMed
  40. Weilhammer D, et al. 2013. Biomaterials. 34:10305. PubMed
  41. Luo N, et al. 2018. Nat Commun. 0.547222222. PubMed
RRID
AB_493524 (BioLegend Cat. No. 107615) AB_493523 (BioLegend Cat. No. 107616)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

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