Alexa Fluor® 594 anti-mouse/human Mac-2 (Galec-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3456	1

Product Details

Verified Reactivity

Mouse, Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Raised against galectin-3 of mouse origin

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

IHC-F - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 10 μg/mL?is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections^3-6, Western blotting², immunoprecipitation^1,2, immunofluorescence^7,8, and ELISA⁹.

Clone M3/38 has been reported to recognize residues 48-100 in the amino-terminal domain of galectin-3⁷.

Application References (PubMed link indicates BioLegend citation)

Ho MK. and Springer TA. 1982. J. Immunol. 128:1221. (FC, IP)
Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731. (WB, IP)
Evans CE, et al. 2010. Arterioscler Vasc Biol. (IHC) PubMed
Jacob N, et al. 2011. J. Immunol. 186:4984. (IHC) PubMed
Li X, et al. 2011. Am J Physiol Heart Circ Physiol. 301:1932. (IHC) PubMed
Chao C, et al. 2012. Clin Cancer Res. 18:4702. (IHC) PubMed
Melo FH, et al. 2011. PLoS One. 6:e29313. (IF)
Usategui A, et al. 2013. Ann Rheum Dis. 72:2018. PubMed (IF)
Mey A, et al. 1996. J. Immunol. 156:1572. (ELISA)
Reales E, et al. 2015. J Cell Sci. 128:2261. PubMed

RRID

AB_2565600 (BioLegend Cat. No. 125412)

Antigen Details

Biology Area: Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules
Antigen References: 1. Ho MK. and Springer TA. 1982. J. Immunol. 128:1221.
2. Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731.
Gene ID: 16854 View all products for this Gene ID 3958 View all products for this Gene ID
UniProt: View information about Mac-2 on UniProt.org

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