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Alexa Fluor® 488 anti-mouse/human Mac-2 (Galec
产品名称:
Alexa Fluor® 488 anti-mouse/human Mac-2 (Galec
产品类别:
抗体
产品编号:
125410
产品应用:
125410
[价格]
规格 价格 库存
100µg ¥ 3180 1

产品详情

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Raised against galectin-3 of mouse origin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections3-6, Western blotting2, immunoprecipitation1,2, immunofluorescence7,8, and ELISA9.

Clone M3/38 has been reported to recognize residues 48-100 in the amino-terminal domain of galectin-37.

Application References

(PubMed link indicates BioLegend citation)
  1. Ho MK. and Springer TA. 1982. J. Immunol. 128:1221. (FC, IP)
  2. Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731. (WB, IP)
  3. Evans CE, et al. 2010. Arterioscler Vasc Biol. (IHC) PubMed
  4. Jacob N, et al. 2011. J. Immunol. 186:4984. (IHC) PubMed
  5. Li X, et al. 2011. Am J Physiol Heart Circ Physiol. 301:1932. (IHC) PubMed
  6. Chao C, et al. 2012. Clin Cancer Res. 18:4702. (IHC) PubMed
  7. Melo FH, et al. 2011. PLoS One. 6:e29313. (IF)
  8. Usategui A, et al. 2013. Ann Rheum Dis. 72:2018. PubMed (IF)
  9. Mey A, et al. 1996. J. Immunol. 156:1572. (ELISA)
  10. Reales E, et al. 2015. J Cell Sci. 128:2261. PubMed
Product Citations
  1. Bussi C, et al. 2022. Nat Commun. 13:7338. PubMed
  2. Blackburn CMR, et al. 2021. Atherosclerosis. 330:76. PubMed
  3. Bussi C, et al. 2018. J Cell Sci. 131. PubMed
  4. Alexander K, et al. 2016. Immunol Cell Biol. 95:7-16. PubMed
RRID
AB_10730886 (BioLegend Cat. No. 125410)

Antigen Details

Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules
Antigen References

1. Ho MK. and Springer TA. 1982. J. Immunol. 128:1221.
2. Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731.

Gene ID
16854 View all products for this Gene ID 3958 View all products for this Gene ID
UniProt
View information about Mac-2 on UniProt.org

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