Purified anti-mouse/rat β-Amyloid, 1-16 Antib-上海雅吉生物科技有限公司

规格	价格	库存
25µL	￥ 1344	1

Product Details

Verified Reactivity

Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

The epitope has been determined to be within residues 10-15 of murine Aβ.

Formulation

Phosphate-buffered solution.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.

Application

WB - Quality tested
ICC, IP, ELISA Detection, ELISA Capture - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.005 - 0.025 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone M3.2 recognizes a rodent sequence of A?, ?-CTF, sAPP-a, and full length APP. Clone 3.2 has negligible cross reactivity with human isoforms of APP and beta amyloid.

Application References (PubMed link indicates BioLegend citation)

Morales-Corraliza J, et al. ?2009. PLoS One 4(9):e7134. (IP, WB, ELISA Detection)
Teich AF, et al. 2013. PLoS One 8(2):e55647, 2013. (ELISA Capture, WB)
Lee Y,?et al. 2017. Neuropharmacology. 117:387-400. (ICC)
Lundgren JL, et al. 2015. J Neurochemistry. 135:606-15. (ICC)

Product Citations

Joly S, et al. 2021. Int J Mol Sci. 22:. PubMed
Miranda AM, et al. 2018. Nat Commun. 9:291. PubMed
Sun J, et al. 2019. Nat Commun. 10:53. PubMed
Daria A, et al. 2017. EMBO J. 10.15252/embj.201694591. PubMed
Tambini MD, et al. 2020. Elife. 9:e52612. PubMed
Socodato R, et al. 2020. Cell Reports. 31(12):107796. PubMed
Gulisano W, et al. 2019. J Neurosci. 39:5986. PubMed
Wang W et al. 2019. EBioMedicine. 42:174-187 . PubMed

RRID

AB_2734556 (BioLegend Cat. No. 805707) AB_2564982 (BioLegend Cat. No. 805701) AB_2564981 (BioLegend Cat. No. 805702)

Antigen Details

Structure

Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.

Distribution

Tissue sources: Primarily nervous system, but also adipose tissue, intestine, muscle.

Distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function

The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.

Biology Area

Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation

Molecular Family

APP/β-Amyloid

Antigen References