Alexa Fluor® 647 anti-mouse CD244.2 (2B4 B6 Al
- 产品名称：
- Alexa Fluor® 647 anti-mouse CD244.2 (2B4 B6 Al
- 产品类别：
- 抗体
- 产品编号：
- 133509
- 产品应用：
- 133509
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- m2B4-Fc
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
- Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
The 2B4 antibody reacts with CD244.2, the 2B4 allloantigen which is expressed on C57BL/6 mice, but not on BALB/c mice.
- Application References
(PubMed link indicates BioLegend citation) -
- Lee KM, et al. 2006. Blood. 107:3181.
- Schatzle JD, et al. 1999. P. Natl Acad Sci USA. 96:3870.
- Stepp SE, et al. 1999. Eur. J. Immunol. 29:2392.
- de la Luz Sierra M, et al. 2010. Blood. 115:3970. PubMed
- Product Citations
-
- Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
- Sugita Y, et al. 2021. Oncol Rep. 45:. PubMed
- RRID
- AB_2072854 (BioLegend Cat. No. 133509) AB_2072853 (BioLegend Cat. No. 133510)
- Structure
- A member of the CD2 subset of the immunoglobulin superfamily (CD2 IgSF).
- Distribution
-
All natural killer (NK) cells, IL-2 activated NK (LAK) cells, NKT cells, and a subset of T lymphocytes.
- Function
- Mediate non-MHC-restricted cytotoxicity.
- Ligand/Receptor
- CD48
- Cell Type
- Dendritic cells, NK cells, NKT cells, T cells
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Brown MH, et al. 1998. J. Exp. Med. 188:2083.
2. Davis SJ, et al. 1996. Immunol Today. 17:177.
3. Kumaresan PR, et al. 2000. Immunogenetics. 51:306.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.
- Gene ID
- 18106 View all products for this Gene ID
- UniProt
- View information about CD244.2 on UniProt.org
