Ultra-LEAF™ Purified anti-mouse CD18 Antibod-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1320	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse CTL glycoprotein

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IP, WB, Block, Stim, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, Western blotting¹, in vitro and in vivo blocking of tumor cells metastasis or stimulation of cell-adhesion^2-6, and immunohistochemical staining⁷ of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 101417-101422).

Application References (PubMed link indicates BioLegend citation)

Sanchez-Madrid, et al. 1983. J. Exp. Med. 158:586. (IP, WB)
Zahalka MA, et al. 1993. J. Immunol. 150:4466. (IP, Block, Stim)
Zahalka MA, et al. 1994. Int. Immunol. 6:917. (Block, Stim)
Ishida Y, et al. 1994. Cell. Immunol. 155:414. (Block, Stim)
Isobe M, et al. 1992. Science 255:1125. (Block, Stim)
Driessens MHE, et al. 1996. J. Leukoc. Biol. 60:758. (Block, Stim)
Barlow SC, et al. 2004. Amer. J. Pathol. 165:1849. (IHC)

Product Citations

Mattei F, et al. 2022. iScience. 25:105110. PubMed

RRID

AB_2832273 (BioLegend Cat. No. 101417) AB_2832274 (BioLegend Cat. No. 101418) AB_2832275 (BioLegend Cat. No. 101419) AB_2832276 (BioLegend Cat. No. 101420) AB_2832277 (BioLegend Cat. No. 101421) AB_2832278 (BioLegend Cat. No. 101422)

Antigen Details

Structure: Integrin family, associates with CD11a, CD11b, CD11c, 95 kD
Distribution: All leukocytes
Function: Adhesion
Ligand/Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, fibrinogen
Cell Type: Leukocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Wilson RW, et al. 1993. J. Immunol. 151:1571.
Gene ID: 16414 View all products for this Gene ID
UniProt: View information about CD18 on UniProt.org

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