Alexa Fluor® 647 anti-mouse CD18 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2748	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse CTL glycoprotein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested
3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L?volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, Western blotting¹, in vitro and in vivo blocking of tumor cells metastasis or stimulation of cell-adhesion^2-6, and immunohistochemical staining⁷ of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 101417-101422).

Application References (PubMed link indicates BioLegend citation)

Sanchez-Madrid, et al. 1983. J. Exp. Med. 158:586. (IP, WB)
Zahalka MA, et al. 1993. J. Immunol. 150:4466. (IP, Block, Stim)
Zahalka MA, et al. 1994. Int. Immunol. 6:917. (Block, Stim)
Ishida Y, et al. 1994. Cell. Immunol. 155:414. (Block, Stim)
Isobe M, et al. 1992. Science 255:1125. (Block, Stim)
Driessens MHE, et al. 1996. J. Leukoc. Biol. 60:758. (Block, Stim)
Barlow SC, et al. 2004. Amer. J. Pathol. 165:1849. (IHC)

RRID

AB_2265032 (BioLegend Cat. No. 101414)

Antigen Details

Structure: Integrin family, associates with CD11a, CD11b, CD11c, 95 kD
Distribution: All leukocytes
Function: Adhesion
Ligand/Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, fibrinogen
Cell Type: Leukocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Wilson RW, et al. 1993. J. Immunol. 151:1571.
Gene ID: 16414 View all products for this Gene ID
UniProt: View information about CD18 on UniProt.org

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