Alexa Fluor® 594 anti-mouse CD18 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3036	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse CTL glycoprotein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

IHC-F - Quality tested
FC, 3D IHC- Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.0 - 5.0 μg/mL?is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L?volume.?For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, Western blotting¹, in vitro and in vivo blocking of tumor cells metastasis or stimulation of cell-adhesion^2-6, and immunohistochemical staining⁷ of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 101417-101422).

Application References (PubMed link indicates BioLegend citation)

Sanchez-Madrid, et al. 1983. J. Exp. Med. 158:586. (IP, WB)
Zahalka MA, et al. 1993. J. Immunol. 150:4466. (IP, Block, Stim)
Zahalka MA, et al. 1994. Int. Immunol. 6:917. (Block, Stim)
Ishida Y, et al. 1994. Cell. Immunol. 155:414. (Block, Stim)
Isobe M, et al. 1992. Science 255:1125. (Block, Stim)
Driessens MHE, et al. 1996. J. Leukoc. Biol. 60:758. (Block, Stim)
Barlow SC, et al. 2004. Amer. J. Pathol. 165:1849. (IHC)

Product Citations

Logan C, Bowen C, and Menko A 2017. Sci Rep. . 10.1038/s41598-017-16456-5. PubMed
Subramanian BC, et al. 2020. J Cell Biol. 219:. PubMed

RRID

AB_2563302 (BioLegend Cat. No. 101416)

Antigen Details

Structure: Integrin family, associates with CD11a, CD11b, CD11c, 95 kD
Distribution: All leukocytes
Function: Adhesion
Ligand/Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, fibrinogen
Cell Type: Leukocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Wilson RW, et al. 1993. J. Immunol. 151:1571.
Gene ID: 16414 View all products for this Gene ID
UniProt: View information about CD18 on UniProt.org

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