Ultra-LEAF™ Purified anti-mouse CD11a Antibo-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1320	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

C57BL/6 mouse splenic secondary cytotoxic T cells

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume or 100 ?l of whole blood.? It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The M17/4 antibody can block CD11a-mediated cellular adhesion. Additional reported applications of this antibody (for the relevant formats) include: immunoprecipitation^1,2, in vitro blocking of cell-cell adhesion^1,2 and FOXP3 expression⁵, and immunohistochemical staining of acetone-fixed frozen sections³. The M17/4 antibody does not block the binding of 2D7 antibody (Cat. No. 101002) to CD11a. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 101110). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 101118) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References (PubMed link indicates BioLegend citation)

Sanchez-Madrid F, et al. 1982. Cell Immunol. 73:1. (IP, Block)
Kuhlman P, et al. 1991. J. Immunol. 146:1773. (IP, Block)
Mizgerd JP, et al. 1997. J. Exp. Med. 186:1357. (IHC)
Hailman E and Allen PM. 2005. J. Immunol. 175:4847. (FC)
Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (Block) PubMed

Product Citations

Bae SH, et al. 2021. Theranostics. 11:2522. PubMed
Wolf D, et al. 2018. Nat Commun. 9:525. PubMed
Waldman MM, et al. 2022. Front Immunol. 13:856977. PubMed

RRID

AB_2819777 (BioLegend Cat. No. 101117) AB_2561478 (BioLegend Cat. No. 101118) AB_2819778 (BioLegend Cat. No. 101127) AB_2819779 (BioLegend Cat. No. 101128) AB_2819780 (BioLegend Cat. No. 101129) AB_2832270 (BioLegend Cat. No. 101130)

Antigen Details

Structure: Integrin family, associates with integrin β2 (CD18), 180 kD
Distribution: Lymphocytes, granulocytes, monocytes, macrophages
Function: Cellular adhesion
Ligand/Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50)
Cell Type: Granulocytes, Lymphocytes, Macrophages, Monocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lub M, et al. 1995. Immunol. Today 16:479.
Gene ID: 16408 View all products for this Gene ID
UniProt: View information about CD11a on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们