PerCP/Cyanine5.5 anti-mouse CD11a Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1548	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

C57BL/6 mouse splenic secondary cytotoxic T cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The M17/4 antibody can block CD11a-mediated cellular adhesion. Additional reported applications of this antibody (for the relevant formats) include: immunoprecipitation^1,2, in vitro blocking of cell-cell adhesion^1,2 and FOXP3 expression⁵, and immunohistochemical staining of acetone-fixed frozen sections³. The M17/4 antibody does not block the binding of 2D7 antibody (Cat. No. 101002) to CD11a. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 101110). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 101118) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Sanchez-Madrid F, et al. 1982. Cell Immunol. 73:1. (IP, Block)
Kuhlman P, et al. 1991. J. Immunol. 146:1773. (IP, Block)
Mizgerd JP, et al. 1997. J. Exp. Med. 186:1357. (IHC)
Hailman E and Allen PM. 2005. J. Immunol. 175:4847. (FC)
Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (Block) PubMed

Product Citations

Cabrera-Mora M, et al. 2016. J Immunol. 197: 2748 - 2761. PubMed
Valanparambil RM, et al. 2017. PLoS Pathog. 13:e1006647. PubMed
Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
Zhao H, et al. 2021. Int J Nanomedicine. 16:4913. PubMed
Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
Zhao H, et al. 2022. Int J Biol Sci. 18:154. PubMed

RRID

AB_2562931 (BioLegend Cat. No. 101123) AB_2562932 (BioLegend Cat. No. 101124)

Antigen Details

Structure: Integrin family, associates with integrin β2 (CD18), 180 kD
Distribution: Lymphocytes, granulocytes, monocytes, macrophages
Function: Cellular adhesion
Ligand/Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50)
Cell Type: Granulocytes, Lymphocytes, Macrophages, Monocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lub M, et al. 1995. Immunol. Today 16:479.
Gene ID: 16408 View all products for this Gene ID
UniProt: View information about CD11a on UniProt.org

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