首页 > 抗体蛋白 > 抗体
Alexa Fluor® 488 anti-mouse CD11a Antibody
产品名称:
Alexa Fluor® 488 anti-mouse CD11a Antibody
产品类别:
抗体
产品编号:
101111
产品应用:
101111
[价格]
规格 价格 库存
25µg ¥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/6 mouse splenic secondary cytotoxic T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Application Notes

The M17/4 antibody can block CD11a-mediated cellular adhesion. Additional reported applications of this antibody (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of cell-cell adhesion1,2 and FOXP3 expression5, and immunohistochemical staining of acetone-fixed frozen sections3. The M17/4 antibody does not block the binding of 2D7 antibody (Cat. No. 101002) to CD11a. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 101110). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 101118) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)
  1. Sanchez-Madrid F, et al. 1982. Cell Immunol. 73:1. (IP, Block)
  2. Kuhlman P, et al. 1991. J. Immunol. 146:1773. (IP, Block)
  3. Mizgerd JP, et al. 1997. J. Exp. Med. 186:1357. (IHC)
  4. Hailman E and Allen PM. 2005. J. Immunol. 175:4847. (FC)
  5. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (Block) PubMed
Product Citations
  1. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
RRID
AB_493432 (BioLegend Cat. No. 101111)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 180 kD
Distribution

Lymphocytes, granulocytes, monocytes, macrophages

Function
Cellular adhesion
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50)
Cell Type
Granulocytes, Lymphocytes, Macrophages, Monocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lub M, et al. 1995. Immunol. Today 16:479.

Gene ID
16408 View all products for this Gene ID
UniProt
View information about CD11a on UniProt.org

联系我们

TEL:021-34661275  点击拨打热线