Ultra-LEAF™ Purified anti-mouse CD14 Antibod
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse CD14 Antibod
- 产品类别：
- 抗体
- 产品编号：
- 150103
- 产品应用：
- 150103
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD14-expressing NRK-52E transfected cells.
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats of this clone) include: blocking.1 M14-23 blocks LPS-binding to CD14/MD4/TLR4 complex in transfected cells.
- Application References
(PubMed link indicates BioLegend citation) -
- Tsukamoto H, et al. 2010. Int. Immunol. 22(4):271. (Block)
- RRID
- AB_2565714 (BioLegend Cat. No. 150103) AB_2565715 (BioLegend Cat. No. 150104)
- Structure
- GPI-linked membrane glycoprotein, 53 - 55 kD.
- Distribution
-
Macrophages, dendritic cells, kupffer cells, and hepatocytes.
- Function
- LPS receptor and clearance of Gram-negative pathogens.
- Ligand/Receptor
- LPS.
- Cell Type
- Macrophages, Dendritic cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Akashi S, et al. 2003. J. Exp. Med. 198:1035.
3. Matsuura K, et al. 1994. J. Exp. Med. 179:1671.
4. Liu S, et al. 1998. Infect. Immun. 66:5089.
- Gene ID
- 12475 View all products for this Gene ID
- UniProt
- View information about CD14 on UniProt.org
