Spark YG™ 593 anti-mouse CD14 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1548	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse CD14-expressing NRK-52E transfected cells.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with Spark YG? 593 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark YG? 593 has a maximum excitation of 573 nm and a maximum emission of 593 nm.

Excitation Laser

Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats of this clone) include: blocking.¹ M14-23 blocks LPS-binding to CD14/MD4/TLR4 complex in transfected cells.

Application References (PubMed link indicates BioLegend citation)

Tsukamoto H, et al. 2010. Int. Immunol. 22(4):271. (Block)

RRID

AB_2894630 (BioLegend Cat. No. 150107) AB_2894630 (BioLegend Cat. No. 150108)

Antigen Details

Structure: GPI-linked membrane glycoprotein, 53 - 55 kD.
Distribution: Macrophages, dendritic cells, kupffer cells, and hepatocytes.
Function: LPS receptor and clearance of Gram-negative pathogens.
Ligand/Receptor: LPS.
Cell Type: Dendritic cells, Macrophages
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Akashi S, et al. 2003. J. Exp. Med. 198:1035.
3. Matsuura K, et al. 1994. J. Exp. Med. 179:1671.
4. Liu S, et al. 1998. Infect. Immun. 66:5089.
Gene ID: 12475 View all products for this Gene ID
UniProt: View information about CD14 on UniProt.org

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