PE anti-mouse CD14 Antibody
- 产品名称：
- PE anti-mouse CD14 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 150105
- 产品应用：
- 150105
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD14-expressing NRK-52E transfected cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats of this clone) include: blocking.1 M14-23 blocks LPS-binding to CD14/MD4/TLR4 complex in transfected cells.
- Application References
(PubMed link indicates BioLegend citation) -
- Tsukamoto H, et al. 2010. Int. Immunol. 22(4):271. (Block)
- Product Citations
-
- Hirakawa MP, et al. 2022. iScience. 25:103759. PubMed
- Li CM, et al. 2020. Cell Reports. 33(13):108566. PubMed
- Gentle IE, et al. 2021. Cell Death Dis. 12:675. PubMed
- Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
- Bae S, et al. 2021. Cell Reports. 35(11):109264. PubMed
- RRID
- AB_2728188 (BioLegend Cat. No. 150105) AB_2728189 (BioLegend Cat. No. 150106)
- Structure
- GPI-linked membrane glycoprotein, 53 - 55 kD.
- Distribution
-
Macrophages, dendritic cells, kupffer cells, and hepatocytes.
- Function
- LPS receptor and clearance of Gram-negative pathogens.
- Ligand/Receptor
- LPS.
- Cell Type
- Macrophages, Dendritic cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Akashi S, et al. 2003. J. Exp. Med. 198:1035.
3. Matsuura K, et al. 1994. J. Exp. Med. 179:1671.
4. Liu S, et al. 1998. Infect. Immun. 66:5089.
- Gene ID
- 12475 View all products for this Gene ID
- UniProt
- View information about CD14 on UniProt.org
