Purified anti-Siglec-E Antibody
- 产品名称：
- Purified anti-Siglec-E Antibody
- 产品类别：
- 抗体
- 产品编号：
- 682802
- 产品应用：
- 682802
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse Siglec-E produced in the 293E cell line.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-F - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody works for Western blotting under non-reducing conditions.
- Application References
(PubMed link indicates BioLegend citation) -
- Siddiqui S, et. al. 2017. J. Biol. Chem. 292: 1029.
- Product Citations
-
- Hsu YW, et al. 2021. J Biomed Sci. 28:5. PubMed
- Siddiqui S, et al. 2016. J Biol Chem. 292:1029-1037. PubMed
- RRID
- AB_2566696 (BioLegend Cat. No. 682802)
- Structure
- 569 amino acids with a predicted molecular weight of approximately 65 kD.
- Distribution
-
Murine Siglec-E is expressed by neutrophils, natural killer (NK) cells, monocytes, macrophages, dendritic cells, and B1 cells.
- Function
- Siglec-E regulates neutrophil recruitment. Siglec-E signaling negatively regulates the LPS-induced production of TNF-α and IL-6 by macrophages. It is upregulated by a variety of TLR agonists.
- Ligand/Receptor
- α2,8-linked disialic acid.
- Cell Type
- B cells, Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Siglec Molecules
- Antigen References
-
1. McMillan SJ, et al. 2013. Blood 121:2084.
2. Bax M, et al. 2007. J. Immunol. 12:8216-24.
3. Angata T, Varki A. 2000. J. Biol. Chem. 275:22127.
4. Zhang JQ, et al. 2004. Eur. J. Immunol. 34:1175.
5. Yu Z, et al. 2001. Biochem. J. 353:483.
- Gene ID
- 83382 View all products for this Gene ID
- UniProt
- View information about Siglec-E on UniProt.org
