Ultra-LEAF? Purified anti-mouse Siglec-E Antibody
- 产品名称：
- Ultra-LEAF? Purified anti-mouse Siglec-E Antibody
- 产品类别：
- 抗体
- 产品编号：
- 934403
- 产品应用：
- 934403
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant Mouse Siglec-E
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by blocking the binding between recombinant a-NEu5Ac-PAA and recombinant mouse Siglec-E-Fc chimera (carrier-free) (Cat. No. 551506). The ND50 range is 0.04 - 0.3 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
- AB_2832884 (BioLegend Cat. No. 934403) AB_2832885 (BioLegend Cat. No. 934404)
- Structure
- Homodimer
- Distribution
-
Murine Siglec-E is expressed by neutrophils, NK cells, monocytes, macrophages, dendritic cells, and B1 cells.
- Function
- Siglec-E regulates neutrophil recruitment. Siglec-E signaling negatively regulates the LPS-induced production of TNF-α and IL-6 by macrophages. It is upregulated by a variety of TLR agonists.
- Ligand/Receptor
- α2, 8-linked disialic acid
- Cell Type
- Embryonic Stem Cells, Mature Neurons, Neural Stem Cells, Neurons, Schwann Cells
- Biology Area
- Cell Biology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Growth Factors
- Antigen References
-
- McMillan SJ, et al. 2013. Blood. 121:2084.
- Bax M,?et al. 2010. Ann. Rheum. Dis. 69:42.
- Angata T and Varki A. 2000. J. Biol.Chem. 275:22127.
- Zhang JQ,?et al. 2004. Eur. J. Immunol. 34:1175.
- Yu Z, et al. 2001. Biochem. J. 353:483.
- Gene ID
- 83382 View all products for this Gene ID
- UniProt
- View information about Siglec-E on UniProt.org
