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Alexa Fluor® 647 anti-mouse/human CD11b Antibo
产品名称：
Alexa Fluor® 647 anti-mouse/human CD11b Antibo
产品类别：
抗体
产品编号：
101220
产品应用：
101220
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse, Human, Cynomolgus, Rhesus
Reported Reactivity
Chimpanzee, Baboon, Rabbit
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume.?For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone M1/70 has been verified for?immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, immunohistochemistry of acetone-fixed frozen sections5,11-13, and spatial biology (IBEX)35,36. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) (Cat. No. 101248).

Application References

(PubMed link indicates BioLegend citation)
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
  2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
  3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
  4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
  7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
  8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
  9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
  10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
  11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
  13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
  14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
  15. Olver S, et al. 2006. Cancer Research 66:571. (FC)
  16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
  17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
  18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
  19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
  21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
  22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
  23. Kim DD, et al. 2008. Blood 112:1109. PubMed
  24. Guo Y, et al. 2008. Blood 112:480. PubMed
  25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed
  26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed
  27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  29. Weber GF, et al. 2014. J Exp Med. 211:1243. PubMed
  30. Ashok A, et al. 2015. Toxicol Sci. 143:64. PubMed
  31. Price PJ, et al. 2015. J Immunol. 194:1164. PubMed
  32. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  33. Ferreira R, et al. 2016. J Infect Dis. 213: 669 - 673. PubMed
  34. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  35. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  36. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Sweet R, et al. 2017. J Immunol. 10.4049/jimmunol.1600861. PubMed
  2. Ortiz G, et al. 2020. Front Immunol. 11:1713. PubMed
  3. Jackson A, et al. 2014. J Leukoc Biol. 92:609. PubMed
  4. Abels ER et al. 2019. Cell Rep. 28(12):3105-3119 . PubMed
  5. Miller EB, et al. 2019. Proc Natl Acad Sci U S A. 116:16603. PubMed
  6. Chen G, et al. 2017. Biosens Bioelectron. 10.1016/j.bios.2016.10.015. PubMed
  7. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  8. Karlen SJ, et al. 2018. J Neuroinflammation. 15:344. PubMed
  9. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  10. Miller EB, et al. 2021. J Neuroinflammation. 18:235. PubMed
  11. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  12. Feng Y, et al. 2018. Kidney Dis (Basel). 4:95. PubMed
  13. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  14. Socodato R, et al. 2020. Sci Signal. 13: . PubMed
  15. Singh A, et al. 2020. Mol Oncol. 1.901388889. PubMed
  16. Wongchana W, et al. 2015. J Immunol. 195: 5337 - 5346. PubMed
  17. Pronin A, et al. 2019. Front Mol Neurosci. 12:36. PubMed
  18. Baxter PS, et al. 2021. Cell Rep. 34:108882. PubMed
  19. Pierce H, et al. 2017. Cell Stem Cell. 1.283333333. PubMed
  20. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  21. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  22. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  23. Szeifert V, et al. 2021. Front Immunol. 12:671995. PubMed
  24. Wei Q, et al. 2020. Dev Cell. 53:503. PubMed
  25. Nowak W, et al. 2020. EBioMedicine. 50:290-305.. PubMed
  26. Cunha LD et al. 2018. Cell. 175(2):429-441 . PubMed
  27. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  28. Laban H, et al. 2018. J Cell Biol. 217:1503. PubMed
  29. Chen ST et al. 2019. Cell host & microbe. 25(4):602-616 . PubMed
  30. Canedo T, et al. 2021. Neuropsychopharmacology. 46:2358. PubMed
  31. Richardson E, et al. 2015. Infect Immun . 83: 2242-2254. PubMed
  32. Sanders K, et al. 2015. Cancer Immunol Res. 3: 891-901. PubMed
  33. Anderson DA, et al. 2022. J Exp Med. 219:. PubMed
  34. Mesa-Nu?ez C, et al. 2022. Mol Ther Methods Clin Dev. 26:459. PubMed
  35. Chen W, et al. 2016. Nat Commun. 7: 11302. PubMed
  36. Bade RM, et al. 2021. Molecular Oncology. . PubMed
  37. Halder LD, et al. 2020. Nat Commun. 2.077083333. PubMed
  38. Catarinella M, et al. 2016. EMBO Mol Med. 8: 155 - 170. PubMed
  39. Oh B, et al. 2017. Plast Reconstr Surg Glob Open. 5:e1595. PubMed
  40. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  41. Cruz F, et al. 2016. Stem Cells Trans Med. 5: 488-499. PubMed
  42. Alberts A, et al. 2020. Front Immunol. 11:596103. PubMed
  43. Kim SI, et al. 2020. Molecular Cancer Therapeutics. 20(1):173-182. PubMed
  44. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  45. Wu L, et al. 2021. Cell Death Dis. 12:1064. PubMed
  46. Rasmussen J, et al. 2006. Infect Immun. 74:6590. PubMed
  47. Maas SLN, et al. 2020. J Neuroinflammation. 17:120. PubMed
RRID
AB_493546 (BioLegend Cat. No. 101220) AB_389327 (BioLegend Cat. No. 101218)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells

Function
Adhesion, chemotaxis
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.

Gene ID
16409 View all products for this Gene ID 3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

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