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首页 > 抗体蛋白 > 抗体
PE/Cyanine7 anti-mouse CD24 Antibody
产品名称：
PE/Cyanine7 anti-mouse CD24 Antibody
产品类别：
抗体
产品编号：
101821
产品应用：
101821
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, in vitro induction of thymocyte maturation2, complement-mediated cytotoxicity3, and immunohistochemistry of acetone-fixed frozen sections4, formalin-fixed paraffin-embedded sections5 and zinc-fixed paraffin-embedded sections10. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 101845 and 101846).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (WB)
  2. Crowley M, et al. 1989. Cell. Immunol. 118:108. (FA)
  3. Veillette A, et al. 1989. J. Exp. Med. 170:1671. (FA)
  4. Pandelakis A Flavell RA 1999 JEM 189:855 (FC, IHC)
  5. Liu JQ, et al. 2007 J. Immunol. 178:6227. (FC, IF)
  6. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  7. Rucci F, et al. 2010. Proc Natl Acad Sci USA. 107:3024. (FC) PubMed
  8. Teague TK, et al. 2010. Int Immunol. 22:387. (FC) PubMed
  9. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (FC) PubMed
  10. Chen CY, et al. 2008. Endocrinology. 10:1210. (FC, IHC) PubMed
  11. Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed
Product Citations
  1. Song W, et al. 2019. Cell Res. 29:206. PubMed
  2. Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed
  3. Schneider C, et al. 2018. Cell. 174:271. PubMed
  4. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  5. Qiu Q, et al. 2010. J Immunol. 184:1681. PubMed
  6. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  7. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  8. Geng A, et al. 2020. eLife. 9:e56434.. PubMed
  9. Privratsky JR, et al. 2018. Am J Physiol Renal Physiol. 315:F682. PubMed
  10. Huang WJ, et al. 2021. Theranostics. 11:5539. PubMed
  11. Lafkas D, et al. 2013. J Cell Biol. 203:47. PubMed
  12. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  13. Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
  14. Kass E, et al. 2016. Nat Commun. 7:13241. PubMed
  15. Wang J, et al. 2021. Cell Reports. 34(13):108897. PubMed
  16. Codina A, et al. 2019. Cell Syst. 8:136. PubMed
  17. Brandi P, et al. 2022. Cell Rep. 38:110184. PubMed
  18. Wang R, et al. 2021. Front Cell Dev Biol. 9:772669. PubMed
  19. Zhou Q, et al. 2014. J Immunol. 193:496. PubMed
  20. Park JY, et al. 2019. Cell Rep. 27:2548. PubMed
  21. Cai C, et al. 2019. Developmental Biology. 458(1):43-51. PubMed
  22. Lu Y, et al. 2018. Stem Cells. 36:1875. PubMed
  23. K?nigsberger S, et al. 2010. PLoS One. 5:e12468. PubMed
  24. Cui Z, et al. 2021. Cell Death Dis. 12:775. PubMed
  25. O'Leary CE, et al. 2021. Curr Protoc. 1:e77. PubMed
  26. Rosser EC, et al. 2020. Cell Metabolism. 31(4):837-851. PubMed
  27. Di Lorenzo A, et al. 2022. Oncoimmunology. 11:2086752. PubMed
  28. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  29. Bird T, et al. 2013. Proc Natl Acad Sci U S A. 110:6542. PubMed
  30. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  31. Mujal AM, et al. 2022. Cancer Immunol Res. 10:403. PubMed
  32. Daynac M, et al. 2016. Stem Cell Reports. 7:735-748. PubMed
  33. Andrés B, et al. 2012. J Immunol. 189:2300. PubMed
  34. Shue YT, et al. 2022. Nat Commun. 13:2690. PubMed
  35. Kojima T, et al. 2016. Sci Rep. 6:36457. PubMed
  36. Zhou C, et al. 2020. Gut Microbes. 12:1782156. PubMed
  37. Huang X, et al. 2021. Immunity. . PubMed
  38. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  39. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  40. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
  41. Sfakianos JP, et al. 2020. Nat Commun. 2.222222222. PubMed
  42. Seong J, et al. 2018. Development. 145:14. PubMed
RRID
AB_756047 (BioLegend Cat. No. 101821) AB_756048 (BioLegend Cat. No. 101822)

Antigen Details

Structure
GPI-linked sialoglycoprotein, 35-45 kD
Distribution

B cells, granulyocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells

Function
Regulates B cell proliferation and differentiation
Ligand/Receptor
P-selectin, CD24
Cell Type
B cells, Dendritic cells, Epithelial cells, Erythrocytes, Granulocytes, Monocytes, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Aigner S, et al. 1997. Blood 89:3385.
3. Hough MR, et al. 1996. J. Immunol. 156:479.
4. Liu Y, et al. 1992. J. Exp. Med. 175:437.

Gene ID
12484 View all products for this Gene ID
UniProt
View information about CD24 on UniProt.org

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