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首页 > 抗体蛋白 > 抗体
Pacific Blue™ anti-mouse CD24 Antibody
产品名称：
Pacific Blue™ anti-mouse CD24 Antibody
产品类别：
抗体
产品编号：
101819
产品应用：
101819
[价格]
规格 价格 库存
25µg ￥ 1128 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, in vitro induction of thymocyte maturation2, complement-mediated cytotoxicity3, and immunohistochemistry of acetone-fixed frozen sections4, formalin-fixed paraffin-embedded sections5 and zinc-fixed paraffin-embedded sections10. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 101845 and 101846).

Application References

(PubMed link indicates BioLegend citation)
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (WB)
  2. Crowley M, et al. 1989. Cell. Immunol. 118:108. (FA)
  3. Veillette A, et al. 1989. J. Exp. Med. 170:1671. (FA)
  4. Pandelakis A Flavell RA 1999 JEM 189:855 (FC, IHC)
  5. Liu JQ, et al. 2007 J. Immunol. 178:6227. (FC, IF)
  6. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  7. Rucci F, et al. 2010. Proc Natl Acad Sci USA. 107:3024. (FC) PubMed
  8. Teague TK, et al. 2010. Int Immunol. 22:387. (FC) PubMed
  9. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (FC) PubMed
  10. Chen CY, et al. 2008. Endocrinology. 10:1210. (FC, IHC) PubMed
  11. Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed
Product Citations
  1. Benitez C, et al. 2014. PLoS Genet. 10:1004645. PubMed
  2. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  3. Isern J, et al. 2010. Blood. 116:3972. PubMed
  4. Sreekumar A et al. 2017. Dev Cell. 43(4):436-448 . PubMed
  5. Hahn J, et al. 2020. Brain Behav Immun. 87:329. PubMed
  6. Lutes LK, et al. 2021. eLife. 10:00. PubMed
  7. Serrano Martinez P, et al. 2022. iScience. 25:105047. PubMed
  8. Peng X, et al. 2020. Cell Death Dis. 1.051388889. PubMed
  9. Slobodin B, et al. 2020. Molecular Cell. 78(3):434-444.e5.. PubMed
  10. Schoenborn AA, et al. 2018. Gut Microbes. 10:45. PubMed
  11. Ghilas S, et al. 2021. iScience. 24:103059. PubMed
  12. Lassen K, et al. 2014. Proc Natl Acad Sci U S A. 111:7741. PubMed
  13. Pal B, et al. 2017. Nat Commun.. 10.1038/s41467-017-01560-x. PubMed
  14. Beyaz S, et al. 2016. Nature. 531:53-58. PubMed
  15. Cai S et al. 2017. Cell stem cell. 20(2):247-260 . PubMed
  16. Shanahan MT, et al. 2021. Am J Physiol Gastrointest Liver Physiol. 321:G668. PubMed
  17. Kim S, et al. 2021. Elife. 10:. PubMed
  18. Roarty K, et al. 2017. Oncogene. 10.1038/onc.2017.206. PubMed
  19. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  20. Mana MD, et al. 2021. Cell Reports. 35(10):109212. PubMed
  21. X X, et al. 2016. J Immunol . 197: 1683-1691. PubMed
  22. Fu NY, et al. 2018. Dev Cell. 47:629. PubMed
  23. Stine RR, et al. 2020. Cell Stem Cell. 25(6):830-845. PubMed
  24. Gracz A, et al. 2010. Am J Physiol Gastrointest Liver Physiol. 298:G590. PubMed
  25. Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed
  26. Roarty K, et al. 2015. J Cell Biol. 208:351. PubMed
  27. King J, et al. 2012. Am J Physiol Gastrointest Liver Physiol. 303:443. PubMed
  28. Pal B, et al. 2021. Breast Cancer Res. 23:69. PubMed
  29. Seeley-Fallen M, et al. 2014. Proc Natl Acad Sci U S A. 111:9881. PubMed
  30. Orhon I, et al. 2021. Autophagy. :1. PubMed
  31. Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed
  32. Gao Y, et al. 2020. Gastroenterology. 1389:158. PubMed
  33. Teague T, et al. 2010. Int Immunol. 1.185416667. PubMed
RRID
AB_572010 (BioLegend Cat. No. 101819) AB_572011 (BioLegend Cat. No. 101820)

Antigen Details

Structure
GPI-linked sialoglycoprotein, 35-45 kD
Distribution

B cells, granulyocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells

Function
Regulates B cell proliferation and differentiation
Ligand/Receptor
P-selectin, CD24
Cell Type
B cells, Dendritic cells, Epithelial cells, Erythrocytes, Granulocytes, Monocytes, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Aigner S, et al. 1997. Blood 89:3385.
3. Hough MR, et al. 1996. J. Immunol. 156:479.
4. Liu Y, et al. 1992. J. Exp. Med. 175:437.

Gene ID
12484 View all products for this Gene ID
UniProt
View information about CD24 on UniProt.org

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