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Brilliant Violet 421™ anti-mouse CD24 Antibo
产品名称：
Brilliant Violet 421™ anti-mouse CD24 Antibo
产品类别：
抗体
产品编号：
101825
产品应用：
101825
[价格]
规格 价格 库存
125µL ￥ 2052 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
?g sizes: 0.2 mg/mL
?L sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the ?g size, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. For immunofluorescent staining using the ?l size, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, in vitro induction of thymocyte maturation2, complement-mediated cytotoxicity3, and immunohistochemistry of acetone-fixed frozen sections4, formalin-fixed paraffin-embedded sections5 and zinc-fixed paraffin-embedded sections10. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 101845 and 101846).

Application References

(PubMed link indicates BioLegend citation)
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (WB)
  2. Crowley M, et al. 1989. Cell. Immunol. 118:108. (FA)
  3. Veillette A, et al. 1989. J. Exp. Med. 170:1671. (FA)
  4. Pandelakis A Flavell RA 1999 JEM 189:855 (FC, IHC)
  5. Liu JQ, et al. 2007 J. Immunol. 178:6227. (FC, IF)
  6. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  7. Rucci F, et al. 2010. Proc Natl Acad Sci USA. 107:3024. (FC) PubMed
  8. Teague TK, et al. 2010. Int Immunol. 22:387. (FC) PubMed
  9. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (FC) PubMed
  10. Chen CY, et al. 2008. Endocrinology. 10:1210. (FC, IHC) PubMed
  11. Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed
Product Citations
  1. Barrett N, et al. 2016. Cell Rep. 16: 1039-1054. PubMed
  2. Flamar AL, et al. 2020. Immunity. 52(4):606-619.e6.. PubMed
  3. Cayre S, et al. 2020. Development. 147:00:00. PubMed
  4. Mansouri N, et al. 2019. JCI Insight. 4:e128060. PubMed
  5. Cui Z, et al. 2022. Bone Res. 10:58. PubMed
  6. Morales RA, et al. 2022. Mucosal Immunol. 15:940. PubMed
  7. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  8. Romagnoli M, et al. 2020. Development. 147:. PubMed
  9. Romagnoli M, et al. 2019. Stem Cell Reports. 1.077083333. PubMed
  10. Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed
  11. Bresson L, et al. 2018. Development. 145. PubMed
  12. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
  13. Smith CA, et al. 2019. JCI Insight. 4. PubMed
  14. Chiche A, et al. 2019. Breast Cancer Res. 21:13. PubMed
  15. Cherukuri A, et al. 2014. J Am Soc Nephrol. 7:1575. PubMed
  16. Rosser EC, et al. 2020. Cell Metabolism. 31(4):837-851. PubMed
  17. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  18. Cayre S, et al. 2020. Development. . PubMed
RRID
AB_10901159 (BioLegend Cat. No. 101825) AB_2563508 (BioLegend Cat. No. 101826)

Antigen Details

Structure
GPI-linked sialoglycoprotein, 35-45 kD
Distribution

B cells, granulyocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells

Function
Regulates B cell proliferation and differentiation
Ligand/Receptor
P-selectin, CD24
Cell Type
B cells, Dendritic cells, Epithelial cells, Erythrocytes, Granulocytes, Monocytes, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Aigner S, et al. 1997. Blood 89:3385.
3. Hough MR, et al. 1996. J. Immunol. 156:479.
4. Liu Y, et al. 1992. J. Exp. Med. 175:437.

Gene ID
12484 View all products for this Gene ID
UniProt
View information about CD24 on UniProt.org

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