Alexa Fluor® 700 anti-mouse CD24 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1212	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting¹, in vitro induction of thymocyte maturation², complement-mediated cytotoxicity³, and immunohistochemistry of acetone-fixed frozen sections⁴, formalin-fixed paraffin-embedded sections⁵ and zinc-fixed paraffin-embedded sections¹⁰. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 101845 and 101846).

Application References (PubMed link indicates BioLegend citation)

Springer T, et al. 1978. Eur. J. Immunol. 8:539. (WB)
Crowley M, et al. 1989. Cell. Immunol. 118:108. (FA)
Veillette A, et al. 1989. J. Exp. Med. 170:1671. (FA)
Pandelakis A Flavell RA 1999 JEM 189:855 (FC, IHC)
Liu JQ, et al. 2007 J. Immunol. 178:6227. (FC, IF)
Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
Rucci F, et al. 2010. Proc Natl Acad Sci USA. 107:3024. (FC) PubMed
Teague TK, et al. 2010. Int Immunol. 22:387. (FC) PubMed
Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (FC) PubMed
Chen CY, et al. 2008. Endocrinology. 10:1210. (FC, IHC) PubMed
Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed

Product Citations

Kumar B, et al. 2020. Haematologica. Online ahead of print:. PubMed
Delgado AC, et al. 2021. Science. 372:1205. PubMed

RRID

AB_2566729 (BioLegend Cat. No. 101835) AB_2566730 (BioLegend Cat. No. 101836)

Antigen Details

Structure: GPI-linked sialoglycoprotein, 35-45 kD
Distribution: B cells, granulyocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells
Function: Regulates B cell proliferation and differentiation
Ligand/Receptor: P-selectin, CD24
Cell Type: B cells, Dendritic cells, Epithelial cells, Erythrocytes, Granulocytes, Monocytes, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Aigner S, et al. 1997. Blood 89:3385.
3. Hough MR, et al. 1996. J. Immunol. 156:479.
4. Liu Y, et al. 1992. J. Exp. Med. 175:437.
Gene ID: 12484 View all products for this Gene ID
UniProt: View information about CD24 on UniProt.org

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