Alexa Fluor® 594 anti-mouse CD24 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3036	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

IHC-F - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: Western blotting¹, in vitro induction of thymocyte maturation², complement-mediated cytotoxicity³, and immunohistochemistry of acetone-fixed frozen sections⁴, formalin-fixed paraffin-embedded sections⁵ and zinc-fixed paraffin-embedded sections¹⁰. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 101845 and 101846).

Application References (PubMed link indicates BioLegend citation)

Springer T, et al. 1978. Eur. J. Immunol. 8:539. (WB)
Crowley M, et al. 1989. Cell. Immunol. 118:108. (FA)
Veillette A, et al. 1989. J. Exp. Med. 170:1671. (FA)
Pandelakis A Flavell RA 1999 JEM 189:855 (FC, IHC)
Liu JQ, et al. 2007 J. Immunol. 178:6227. (FC, IF)
Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
Rucci F, et al. 2010. Proc Natl Acad Sci USA. 107:3024. (FC) PubMed
Teague TK, et al. 2010. Int Immunol. 22:387. (FC) PubMed
Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (FC) PubMed
Chen CY, et al. 2008. Endocrinology. 10:1210. (FC, IHC) PubMed
Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed

RRID

AB_2565427 (BioLegend Cat. No. 101834)

Antigen Details

Structure: GPI-linked sialoglycoprotein, 35-45 kD
Distribution: B cells, granulyocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells
Function: Regulates B cell proliferation and differentiation
Ligand/Receptor: P-selectin, CD24
Cell Type: B cells, Dendritic cells, Epithelial cells, Erythrocytes, Granulocytes, Monocytes, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Aigner S, et al. 1997. Blood 89:3385.
3. Hough MR, et al. 1996. J. Immunol. 156:479.
4. Liu Y, et al. 1992. J. Exp. Med. 175:437.
Gene ID: 12484 View all products for this Gene ID
UniProt: View information about CD24 on UniProt.org

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