Purified anti-human CD27 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1356	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human T cells from a T-ALL patient.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
IHC-P, ICC, ELISA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections¹, immunofluorescent staining², and ELISA³.

Application References (PubMed link indicates BioLegend citation)

Ma S, et al. 2011. J. Virol. 85:165. (IHC)
Manzo A, et al. 2008. Arthritis Rheum. 11:3377. (IF)
Kato K, et al. 2007. Exp. Hematol. 35:434. (ELISA)

Product Citations

Mann ER, et al. 2020. Sci Immunol. :5. PubMed
Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed
Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed
Klymenko T, et al. 2018. Leukemia. 19:E499. PubMed
Hunter S, et al. 2018. J Hepatol. 69:654. PubMed

RRID

AB_2561786 (BioLegend Cat. No. 356401)

Antigen Details

Structure: TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
Distribution: Medullary thymocytes, T and B cell subsets, NK cells
Interaction: CD27 binds to CD70
Ligand/Receptor: CD70
Cell Type: B cells, NK cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Hintzen R, et al. 1994. Immunol. Today 15:307.
4. Agematsu K, et al. 1995. J. Immunol. 154:3627.
Gene ID: 939 View all products for this Gene ID
UniProt: View information about CD27 on UniProt.org

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