Purified anti-human CD25 Antibody
- 产品名称：
- Purified anti-human CD25 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 356101
- 产品应用：
- 356101
- Verified Reactivity
- Human
- Reported Reactivity
- Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human PHA-induced lymphocyte cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections1?and spatial biology (IBEX)2,3.
The CD25 molecule reveals three epitope regions: A, B, and C. M-A251 antibody recognizes epitope region B. Unlike other CD25 antibody clones, M-A251 can detect CD25 after fixation with paraformaldehyde.
- Application References
(PubMed link indicates BioLegend citation) -
- Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed
- Arunachalam PS, et al. 2021. Nature. 596:410. PubMed
- Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
- Neidleman J, et al. 2020. Elife. 9:00. PubMed
- Neidleman J, et al. 2020. Cell Rep Med. 100081:1. PubMed
- Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
- Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
- James KR, et al. 2020. Nat Immunol. 1.113194444. PubMed
- Neidleman J, et al. 2021. Elife. 10:. PubMed
- Neidleman J, et al. 2021. Cell Rep. 36:109414. PubMed
- Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
- NULL, et al. 2022. Cell. 185:916. PubMed
- Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed
- Joas S, et al. 2020. Cell Rep. 30:2261. PubMed
- Kasatskaya SA, et al. 2020. Elife. :9. PubMed
- Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
- RRID
- AB_2561751 (BioLegend Cat. No. 356101) AB_2561752 (BioLegend Cat. No. 356102)
- Structure
- Type I transmembrane glycoprotein, 55 kD; low-affinity IL-2 receptor α chain
- Distribution
-
Activated T and B cells, monocytes/macrophages, Tregs
- Interaction
- Associates with IL-2Rβ/CD122 and IL-2Rγ/CD132 receptor chains to form a high-affinity IL-2R complex
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Barclay N, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press Inc.
4. Taniguchi T and Minami Y. et al. 1993. Cell 73:5.
5. Waldmann T. 1991. J. Biol. Chem. 266:2681.
- Gene ID
- 3559 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
