PE/Cyanine7 anti-human CD25 Antibody
- 产品名称：
- PE/Cyanine7 anti-human CD25 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 356107
- 产品应用：
- 356107
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1800 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human PHA-induced lymphocyte cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections1?and spatial biology (IBEX)2,3.
The CD25 molecule reveals three epitope regions: A, B, and C. M-A251 antibody recognizes epitope region B. Unlike other CD25 antibody clones, M-A251 can detect CD25 after fixation with paraformaldehyde.
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Nagle VL, et al. 2022. Mol Cancer Ther. 21:658. PubMed
- Kimura I, et al. 2022. Cell Rep. 38:110218. PubMed
- Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
- Martrus G, et al. 2017. PLoS One.. 10.1371/journal.pone.0182532. PubMed
- Cappellano G, et al. 2018. PLoS One. 13:e0192108. PubMed
- Kreutmair S, et al. 2021. Immunity. . PubMed
- Mederacke YS, et al. 2019. Sci Rep. 1.084027778. PubMed
- Studniberg SI, et al. 2022. Mol Syst Biol. 18:e10824. PubMed
- Rasmussen TA, et al. 2022. Cell Rep Med. 3:100766. PubMed
- Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
- Motozono C, et al. 2021. Cell Host Microbe. . PubMed
- Demaria O, et al. 2022. Cell Rep Med. 3:100783. PubMed
- Jin J, et al. 2014. PLoS One. 9:104753. PubMed
- Toor SM, et al. 2018. Clin Exp Immunol. 191:189. PubMed
- RRID
- AB_2561974 (BioLegend Cat. No. 356107) AB_2561975 (BioLegend Cat. No. 356108)
- Structure
- Type I transmembrane glycoprotein, 55 kD; low-affinity IL-2 receptor α chain
- Distribution
-
Activated T and B cells, monocytes/macrophages, Tregs
- Interaction
- Associates with IL-2Rβ/CD122 and IL-2Rγ/CD132 receptor chains to form a high-affinity IL-2R complex
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Barclay N, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press Inc.
4. Taniguchi T and Minami Y. et al. 1993. Cell 73:5.
5. Waldmann T. 1991. J. Biol. Chem. 266:2681.
- Gene ID
- 3559 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
