PE anti-human CD25 Antibody
- 产品名称：
- PE anti-human CD25 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 356103
- 产品应用：
- 356103
- Verified Reactivity
- Human
- Reported Reactivity
- Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human PHA-induced lymphocyte cells
- Formulation
- ?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). - Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- ?g sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.4 ?g per million cells in 100 ?l volume. For flow cytometric staining using tests sizes, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections1?and spatial biology (IBEX)2,3.
The CD25 molecule reveals three epitope regions: A, B, and C. M-A251 antibody recognizes epitope region B. Unlike other CD25 antibody clones, M-A251 can detect CD25 after fixation with paraformaldehyde.
- Application References
(PubMed link indicates BioLegend citation) -
- Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed
- Serra–Peinado C, et al. 2019. Nat Commun. 10:3705. PubMed
- Schmitz T, et al. 2021. J Reprod Immunol. 148:103424. PubMed
- Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
- Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed
- Holbrook AK, et al. 2019. Physiol Rep. 7:e14234. PubMed
- Maritz MF, et al. 2018. PLoS One. 13:e0196043. PubMed
- Ackerley CG, et al. 2022. Front Immunol. 13:972170. PubMed
- Berezhnoy A, et al. 2020. Cell Rep Med. 1:100163. PubMed
- Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
- Tai YT, et al. 2019. Leukemia. 33:426. PubMed
- Bossini-Castillo L, et al. 2022. Cell Genom. 2:None. PubMed
- Waddington KE, et al. 2020. Front Immunol. 1.51875. PubMed
- Yang YK, et al. 2021. Stem Cell Res Ther. 12:156. PubMed
- Abd Hamid M et al. 2019. Cancer Immunol Res. 7(8):1293-1306 . PubMed
- Bassaganyas L, et al. 2019. J Cell Biol. 218:3861. PubMed
- Metz PJ, et al. 2020. Cell Reports. 30(6):1935-1950. PubMed
- Gamradt S, et al. 2021. iScience. 24:103312. PubMed
- Lake CM, et al. 2021. Cell Death Dis. 12:400. PubMed
- Hoseini SS, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(5):. PubMed
- RRID
- AB_2561860 (BioLegend Cat. No. 356103) AB_2561861 (BioLegend Cat. No. 356104) AB_2564145 (BioLegend Cat. No. 356134)
- Structure
- Type I transmembrane glycoprotein, 55 kD; low-affinity IL-2 receptor α chain
- Distribution
-
Activated T and B cells, monocytes/macrophages, Tregs
- Interaction
- Associates with IL-2Rβ/CD122 and IL-2Rγ/CD132 receptor chains to form a high-affinity IL-2R complex
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Barclay N, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press Inc.
4. Taniguchi T and Minami Y. et al. 1993. Cell 73:5.
5. Waldmann T. 1991. J. Biol. Chem. 266:2681.
- Gene ID
- 3559 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
