PE/Dazzle™ 594 anti-mouse CD301 (MGL1/MGL2)
- 产品名称：
- PE/Dazzle™ 594 anti-mouse CD301 (MGL1/MGL2)
- 产品类别：
- 抗体
- 产品编号：
- 145713
- 产品应用：
- 145713
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Purified and recombinant mouse MGL1 and MGL2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
LOM-14 recognizes mouse CD301a and CD301b. Additional reported applications (for relevant formats) include: immunohistochemical staining of frozen tissue sections1-3 and immunoprecipitation4.
- Application References
(PubMed link indicates BioLegend citation) -
- Denda-Nagai K, et al. 2010. J. Biol. Chem. 285:19193. (IHC)
- Sato K, et al. 2005. Blood 106:207. (IHC)
- Tsuiji M, et al. 2002. J. Biol. Chem. 277:28892. (IHC)
- Kimura T, et al. 1995. J. Biol. Chem. 270:16056. (IP)
- RRID
- AB_2819895 (BioLegend Cat. No. 145713) AB_2819896 (BioLegend Cat. No. 145714)
- Structure
- 42 kD type II transmembrane glycoprotein, contain a cytoplasmic domain, a transmembrane domain, a neck domain, and a carbohydrate recognition domain (CRD)
- Distribution
-
Immature dendritic cells, a subset of macrophages
- Function
- Cell adhesion, cell-cell signaling, glycoprotein turnover, inflammation, tissue remodeling
- Interaction
- Carbohydrate determinants, GP envelope glycoprotein on Marburg and Ebola viruses
- Ligand/Receptor
- Glycans containing terminal GalNAc moieties
- Cell Type
- Dendritic cells, Macrophages
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Denda-Nagai K,?et al. 2010. J. Biol. Chem. 285:19193.
2. Westcott D, et al. 2009. J. Exp. Med. 206:3143.
3. Singh SK, et al. 2009. Mol. Immunol. 46:1240.
4. Sakakura M, et al. 2008. J. Biol. Chem. 283:33665.
5. Tsuiji M, et al. 2002. J. Biol. Chem. 277:28892.
- Gene ID
- 17312 View all products for this Gene ID 216864 View all products for this Gene ID
- UniProt
- View information about CD301 on UniProt.org
