Ultra-LEAF™ Purified anti-mouse/rat/human CD-上海雅吉生物科技有限公司

规格	价格	库存
1mg	￥ 8124	1

Product Details

Verified Reactivity

Mouse, Rat, Human

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: functional assay^1,3 and immunohistomecial staining of acetone-fixed frozen sections². The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 124243 & 124244).

Application References (PubMed link indicates BioLegend citation)

Gravestein LA, et al. 1995. Int. Immunol. 7:551. (FA)
Gravestein LA, et al. 1996. J. Exp. Med. 184:675. (IHC)
Takeda K, et al. 2000. J. Immunol. 164:1741. (FA)
Welner RS, et al. 2009. J. Immunol. 183:7768. PubMed
Vicetti Miguel RC, et al. 2012. J. Immunol. 189:3449. PubMed
White CA, et al. 2014. J Immunol. 193:5933. PubMed
Iwata S, et al. 2015. Lupus. 24:695. PubMed

RRID

AB_2813994 (BioLegend Cat. No. 124243) AB_2813995 (BioLegend Cat. No. 124244)

Antigen Details

Structure: TNFR superfamily, 45 kD
Distribution: Peripheral blood T cells, memory B cells, NK cells, thymocyte subset
Function: Costimulatory signal for T and B cell activation, T cell development
Interaction: TRAF2, TRAF5, CD27-binding protein (Siva)
Ligand/Receptor: CD70
Cell Type: B cells, NK cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Hintzen RQ, et al. 1994. Immunol. Today 15:307.
2. Akiba H, et al. 1998. J. Biol. Chem. 273:13353.
3. Kobata T, et al. 1995. P. Natl. Acad. Sci. USA 92:11249.
4. Hendriks J, et al. 2000. Nat. Immunol. 1:433
5. Hintzen RQ, et al. 1994. Int. Immunol. 6:477
6. Camerini D, et al. 1991. J. Immunol. 147:3165
Gene ID: 21940 View all products for this Gene ID 939 View all products for this Gene ID 500318 View all products for this Gene ID
UniProt: View information about CD27 on UniProt.org

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