Purified anti-mouse/rat/human CD27 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
500µg	￥ 3096	1

Product Details

Verified Reactivity

Mouse, Rat, Human

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA

Formulation

Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C."

Application

FC - Quality tested
IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: functional assay^1,3 and immunohistomecial staining of acetone-fixed frozen sections². The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 124243 & 124244).

Application References (PubMed link indicates BioLegend citation)

Gravestein LA, et al. 1995. Int. Immunol. 7:551. (FA)
Gravestein LA, et al. 1996. J. Exp. Med. 184:675. (IHC)
Takeda K, et al. 2000. J. Immunol. 164:1741. (FA)
Welner RS, et al. 2009. J. Immunol. 183:7768. PubMed
Vicetti Miguel RC, et al. 2012. J. Immunol. 189:3449. PubMed
White CA, et al. 2014. J Immunol. 193:5933. PubMed
Iwata S, et al. 2015. Lupus. 24:695. PubMed

Product Citations

Dienz O, et al. 2020. J Immunol. 204:1521. PubMed
?lvarez-Salamero C, et al. 2020. PLoS Biol. 18:e3000646. PubMed
Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
Mouat IC, et al. 2021. Elife. 10:. PubMed
Wei SC, et al. 2019. Immunity. 50:1084. PubMed
Tao H, et al. 2021. Front Immunol. 12:623280. PubMed
Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
Deng Y, et al. 2021. Blood. 137:3225. PubMed
Brownlie D, et al. 2021. J Immunother Cancer. 9:. PubMed
Goltsev Y et al. 2018. Cell. 174(4):968-981 . PubMed
Martin E, et al. 2020. JCI Insight. :5. PubMed

RRID

AB_1236456 (BioLegend Cat. No. 124202)

Antigen Details

Structure: TNFR superfamily, 45 kD
Distribution: Peripheral blood T cells, memory B cells, NK cells, thymocyte subset
Function: Costimulatory signal for T and B cell activation, T cell development
Interaction: TRAF2, TRAF5, CD27-binding protein (Siva)
Ligand/Receptor: CD70
Cell Type: B cells, NK cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Hintzen RQ, et al. 1994. Immunol. Today 15:307.
2. Akiba H, et al. 1998. J. Biol. Chem. 273:13353.
3. Kobata T, et al. 1995. P. Natl. Acad. Sci. USA 92:11249.
4. Hendriks J, et al. 2000. Nat. Immunol. 1:433
5. Hintzen RQ, et al. 1994. Int. Immunol. 6:477
6. Camerini D, et al. 1991. J. Immunol. 147:3165
Gene ID: 21940 View all products for this Gene ID 939 View all products for this Gene ID 500318 View all products for this Gene ID
UniProt: View information about CD27 on UniProt.org

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