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PerCP/Cyanine5.5 anti-mouse/rat/human CD27 Antibod
产品名称：
PerCP/Cyanine5.5 anti-mouse/rat/human CD27 Antibod
产品类别：
抗体
产品编号：
124213
产品应用：
124213
[价格]
规格 价格 库存
25µg ￥ 1416 1

产品详情

Product Details

Verified Reactivity
Mouse, Rat, Human
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA
Formulation
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: functional assay1,3 and immunohistomecial staining of acetone-fixed frozen sections2. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 124243 & 124244).

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Gravestein LA, et al. 1995. Int. Immunol. 7:551. (FA)
  2. Gravestein LA, et al. 1996. J. Exp. Med. 184:675. (IHC)
  3. Takeda K, et al. 2000. J. Immunol. 164:1741. (FA)
  4. Welner RS, et al. 2009. J. Immunol. 183:7768. PubMed
  5. Vicetti Miguel RC, et al. 2012. J. Immunol. 189:3449. PubMed
  6. White CA, et al. 2014. J Immunol. 193:5933. PubMed
  7. Iwata S, et al. 2015. Lupus. 24:695. PubMed
Product Citations
  1. Rezende R, et al. 2015. Nat Commun. 6: 8726. PubMed
  2. Wang D, et al. 2018. Immunity. 48:659. PubMed
  3. Sandrock I, et al. 2015. PLoS One. 10: 0145010. PubMed
  4. Stotesbury C, et al. 2020. J Immunol. 204:1582. PubMed
  5. Gu X, et al. 2022. Vaccines (Basel). 10:. PubMed
  6. De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
  7. Colston J, et al. 2016. J Immunol. 196: 3354-3363. PubMed
  8. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  9. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
  10. Topham H 2010. J Virol. 84:7750. PubMed
  11. S Tzeng 2016. J Vis Exp. 118:e54582. PubMed
  12. Bantug GR, et al. 2018. Immunity. 48:542. PubMed
  13. Rissiek B, et al. 2014. J Leukoc Biol. 95:543. PubMed
  14. Forconi F, et al. 2010. Blood. 115:71. PubMed
RRID
AB_2073424 (BioLegend Cat. No. 124213) AB_2275577 (BioLegend Cat. No. 124214)

Antigen Details

Structure
TNFR superfamily, 45 kD
Distribution

Peripheral blood T cells, memory B cells, NK cells, thymocyte subset

Function
Costimulatory signal for T and B cell activation, T cell development
Interaction
TRAF2, TRAF5, CD27-binding protein (Siva)
Ligand/Receptor
CD70
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Hintzen RQ, et al. 1994. Immunol. Today 15:307.
2. Akiba H, et al. 1998. J. Biol. Chem. 273:13353.
3. Kobata T, et al. 1995. P. Natl. Acad. Sci. USA 92:11249.
4. Hendriks J, et al. 2000. Nat. Immunol. 1:433
5. Hintzen RQ, et al. 1994. Int. Immunol. 6:477
6. Camerini D, et al. 1991. J. Immunol. 147:3165

Gene ID
21940 View all products for this Gene ID 939 View all products for this Gene ID 500318 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org

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