PE/Cyanine7 anti-mouse/rat/human CD27 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1416	1

Product Details

Verified Reactivity

Mouse, Rat, Human

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA

Formulation

Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume.? It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: functional assay^1,3 and immunohistomecial staining of acetone-fixed frozen sections². The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 124243 & 124244).

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Gravestein LA, et al. 1995. Int. Immunol. 7:551. (FA)
Gravestein LA, et al. 1996. J. Exp. Med. 184:675. (IHC)
Takeda K, et al. 2000. J. Immunol. 164:1741. (FA)
Welner RS, et al. 2009. J. Immunol. 183:7768. PubMed
Vicetti Miguel RC, et al. 2012. J. Immunol. 189:3449. PubMed
White CA, et al. 2014. J Immunol. 193:5933. PubMed
Iwata S, et al. 2015. Lupus. 24:695. PubMed

Product Citations

Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
Verma S, et al. 2016. J Virol. 90: 650 - 658. PubMed
Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
Mancini M, et al. 2019. J Immunol. 202:1479. PubMed
Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed
Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed
Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
Crinier A, et al. 2018. Immunity. 49:971. PubMed
Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
Chakraborty A, et al. 2022. Methods Mol Biol. 2442:565. PubMed
Jin F, et al. 2019. Oncol Lett. 18:2394. PubMed

RRID

AB_10645330 (BioLegend Cat. No. 124215) AB_10639726 (BioLegend Cat. No. 124216)

Antigen Details

Structure: TNFR superfamily, 45 kD
Distribution: Peripheral blood T cells, memory B cells, NK cells, thymocyte subset
Function: Costimulatory signal for T and B cell activation, T cell development
Interaction: TRAF2, TRAF5, CD27-binding protein (Siva)
Ligand/Receptor: CD70
Cell Type: B cells, NK cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Hintzen RQ, et al. 1994. Immunol. Today 15:307.
2. Akiba H, et al. 1998. J. Biol. Chem. 273:13353.
3. Kobata T, et al. 1995. P. Natl. Acad. Sci. USA 92:11249.
4. Hendriks J, et al. 2000. Nat. Immunol. 1:433
5. Hintzen RQ, et al. 1994. Int. Immunol. 6:477
6. Camerini D, et al. 1991. J. Immunol. 147:3165
Gene ID: 21940 View all products for this Gene ID 939 View all products for this Gene ID 500318 View all products for this Gene ID
UniProt: View information about CD27 on UniProt.org

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