Brilliant Violet 421™ anti-mouse/rat/human C-上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 3360	1

Product Details

Verified Reactivity

Mouse, Rat, Human

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: functional assay^1,3 and immunohistomecial staining of acetone-fixed frozen sections². The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 124243 & 124244).

Application References (PubMed link indicates BioLegend citation)

Gravestein LA, et al. 1995. Int. Immunol. 7:551. (FA)
Gravestein LA, et al. 1996. J. Exp. Med. 184:675. (IHC)
Takeda K, et al. 2000. J. Immunol. 164:1741. (FA)
Welner RS, et al. 2009. J. Immunol. 183:7768. PubMed
Vicetti Miguel RC, et al. 2012. J. Immunol. 189:3449. PubMed
White CA, et al. 2014. J Immunol. 193:5933. PubMed
Iwata S, et al. 2015. Lupus. 24:695. PubMed

Product Citations

Sagnella SM, et al. 2020. Cancer Cell. 37(3):354-370. PubMed
Flommersfeld S, et al. 2021. Immunity. :. PubMed
Snell LM, et al. 2018. Immunity. 49:678. PubMed
Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
Dupraz L, et al. 2021. Cell Reports. 36(1):109332. PubMed

RRID

AB_2565547 (BioLegend Cat. No. 124223)

Antigen Details

Structure: TNFR superfamily, 45 kD
Distribution: Peripheral blood T cells, memory B cells, NK cells, thymocyte subset
Function: Costimulatory signal for T and B cell activation, T cell development
Interaction: TRAF2, TRAF5, CD27-binding protein (Siva)
Ligand/Receptor: CD70
Cell Type: B cells, NK cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Hintzen RQ, et al. 1994. Immunol. Today 15:307.
2. Akiba H, et al. 1998. J. Biol. Chem. 273:13353.
3. Kobata T, et al. 1995. P. Natl. Acad. Sci. USA 92:11249.
4. Hendriks J, et al. 2000. Nat. Immunol. 1:433
5. Hintzen RQ, et al. 1994. Int. Immunol. 6:477
6. Camerini D, et al. 1991. J. Immunol. 147:3165
Gene ID: 21940 View all products for this Gene ID 939 View all products for this Gene ID 500318 View all products for this Gene ID
UniProt: View information about CD27 on UniProt.org

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