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Purified anti-Cytokeratin 18 Antibody
产品名称:
Purified anti-Cytokeratin 18 Antibody
产品类别:
抗体
产品编号:
689102
产品应用:
689102
[价格]
规格 价格 库存
100µg ¥ 2616 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide of human keratin 18 (CSETNDTKVLRH-COOH).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
IHC-P - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125 - 0.5 ?g/ml (1:1000 - 1:4000 dilution). For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry1 on formalin-fixed paraffin-embedded tissue (IHC-P).
?

This clone and BioLegend clone DA-7 displayed similar affinities when tested for western blot applications

Application References

(PubMed link indicates BioLegend citation)
  1. Hosoya A. et al. 2010. Anat. Rec. (Hoboken) 293:1864. (IHC-P)
RRID
AB_2629745 (BioLegend Cat. No. 689102)

Antigen Details

Structure
Type I intermediate filament protein, contains three coiled-coil domains, approximately 48 kD. Heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 associates with cytokeratin 8.
Distribution

Keratin 18 is widely expressed in normal single layer epithelial tissues (liver, pancreas) and is expressed at high levels in some tumor tissues.

Function
Intermediate filament protein involved with the cytoskeleton.
Interaction
Interacts with a number of proteins including caspase3, 14-3-3 gamma, 14-3-3 zeta, 14-3-3 sigma 14-3-3 beta, 14-3-3 eta, keratin 5, keratin 8, TNF receptor II, plakophilin 2, EGF receptor, usherin.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Ku N, et al. 2003. Proc. Natl. Acad. Sci. USA 100:6063.
2. Kulesh DA, Oshima RG. 1989. Genomics 4:339.
3. Steinert PM, Roop DR. 1988. Ann. Rev. Biochem. 57:593.
4. Izawa I, et al. 2000. J. Biol. Chem. 275:34521.
5. Pankov R, et al. 1994. Proc. Natl. Acad. Sci. USA 91:873.

Gene ID
3875 View all products for this Gene ID
UniProt
View information about Cytokeratin 18 on UniProt.org

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