Purified anti-Lamin A/C Antibody
- 产品名称：
- Purified anti-Lamin A/C Antibody
- 产品类别：
- 抗体
- 产品编号：
- 615801
- 产品应用：
- 615801
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- GST fused to C-terminal fragment of Lamin A (amino acid residues 422-664)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
LASS2D9 recognized both human and mouse Lamin A/C when tested by WB; WL4G10 does not recognize mouse Lamin A or C.
WL4G10 displayed a significantly higher affinity for human Lamin A/C compared to LASS2D9 when tested for WB.
During PD testing for ICC applications, this clone produced weak staining of PFA-fixed HeLa cells that had been permeabilized with methanol or Triton X-100. We therefore do not recommend this clone for the application.
- RRID
- AB_2810663 (BioLegend Cat. No. 615801) AB_2810664 (BioLegend Cat. No. 615802)
- Structure
- Lamin A is a 664 amino acid protein with a predicted molecular weight of 74 kD. Lamin C is a 572 amino acid protein with a predicted molecular weight of 65 kD.
- Distribution
-
Ubiquitously expressed/Nucleoplasmic side of inner nuclear membrane
- Function
- Fibrous component of nuclear lamina, provides framework for nuclear envelope, may interact with chromatin
- Cell Type
- Neurons
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Nuclear Markers
- Antigen References
-
- Machiels B, et al. 1996. J. Biol. Chem. 271:9249.
- Barton R and Worman HJ. 1999. J. Biol. Chem. 274:30008.
- Gruenbaum Y, et al. 2000. J. Struct. Biol. 129:313.
- Stierlé V, et al. 2003. Biochemistry 42:4819.
- Gene ID
- 4000 View all products for this Gene ID
- UniProt
- View information about Lamin A/C on UniProt.org
