Ultra-LEAF™ Purified anti-DYKDDDDK Tag Antib-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2772	1

Product Details

Verified Reactivity

DYKDDDDK tag epitope

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

DYKDDDDK-tagged mouse Langerin

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

WB - Quality tested
IP, ICC, ELISA, FC, Purification - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 ?g per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.

Application References (PubMed link indicates BioLegend citation)

Park SH, et al. 2008. J Immunol Methods. 331:27.
Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed

RRID

AB_2810691 (BioLegend Cat. No. 637327) AB_2810692 (BioLegend Cat. No. 637328)

Antigen Details

Biology Area: Cell Biology
Antigen References: 1. Einhauer A. 2001. J. Biochem. Biophys. Methods. 49:455.
2. Knappik A and Pluckthun A. 1994. Biotechniques. 17:754.
Gene ID: NA
UniProt: View information about DYKDDDDK on UniProt.org

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