Direct-Blot™ HRP anti-DYKDDDDK Tag Antibody
- 产品名称：
- Direct-Blot™ HRP anti-DYKDDDDK Tag Antibody
- 产品类别：
- 抗体
- 产品编号：
- 637312
- 产品应用：
- 637312
- Verified Reactivity
- DYKDDDDK tag epitope
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- DYKDDDDK-tagged mouse Langerin
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.
25 ?l and 100 ?l of Direct-Blot? HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
- Application Notes
The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.
- Application References
(PubMed link indicates BioLegend citation) -
- Park SH, et al. 2008. J Immunol Methods. 331:27.
- Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
- Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
- Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
- Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
- Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
- Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed
- Product Citations
-
- Zavaliev R, et al. 2020. Cell. 182(5):1093-1108.e18. PubMed
- Qu Y, et al. 2021. Front Cell Dev Biol. 9:716208. PubMed
- Taverner WK, et al. 2019. Mol Ther Oncolytics. 0.70625. PubMed
- Liu Z, et al. 2021. PLoS Pathog. 17:e1010123. PubMed
- Uche UU, et al. 2018. J Exp Med. 215:3165. PubMed
- Greulich W, et al. 2020. Cell. 179(6):1264-1275.e13.. PubMed
- RRID
- AB_2734514 (BioLegend Cat. No. 637312) AB_2566706 (BioLegend Cat. No. 637311)
