Alexa Fluor® 488 anti-DYKDDDDK Tag Antibody
- 产品名称：
- Alexa Fluor® 488 anti-DYKDDDDK Tag Antibody
- 产品类别：
- 抗体
- 产品编号：
- 637317
- 产品应用：
- 637317
- Verified Reactivity
- DYKDDDDK tag epitope
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- DYKDDDDK-tagged mouse Langerin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250 - 1:1000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.
- Application References
(PubMed link indicates BioLegend citation) -
- Park SH, et al. 2008. J Immunol Methods. 331:27.
- Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
- Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
- Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
- Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
- Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
- Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed
- Product Citations
-
- Wang J, et al. 2022. Front Immunol. 13:863346. PubMed
- Jayasinghe MK, et al. 2022. Theranostics. 12:3288. PubMed
- Martin-Sancho L, et al. 2021. Mol Cell. 81:2656. PubMed
- Martin-Sancho L, et al. 2020. bioRxiv. . PubMed
- Gallo E, et al. 2020. MAbs. 12:1717265. PubMed
- RRID
- AB_2728470 (BioLegend Cat. No. 637317) AB_2810690 (BioLegend Cat. No. 637318)
