GoInVivo™ Purified anti-human CD152 (CTLA-4)-上海雅吉生物科技有限公司

规格	价格	库存
5mg	￥ 9240	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Extracellular domain of human CTLA-4 and human IgG1 Fc fusion protein

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <1.0 EU/mg of the protein. (<0.1 pg/?g of the protein) as determined by the LAL test.

Preparation

The GoInVivo? antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo? solution contains no preservative; handle under aseptic conditions.

Application

ICFC - Quality tested
Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.

Application Notes

GoInVivo? products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo? Webpage.

ELISA Detection: The biotinylated L3D10 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified A3.6B10.G1 antibody (Cat. No. 525401) as the capture antibody and recombinant human CTLA-4 (Cat. No. 591909) as the standard.

Flow Cytometry: The fluorochrome-labeled L3D10 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify CTLA-4-producing cells within mixed cell populations.

Note: For testing human soluble CTLA-4 in serum, plasma or cell culture supernatant, LEGEND MAX? Human Soluble CTLA-4 ELISA Kit with Pre-coated Plates (Cat. No. 437407 & 437408) are specially developed and recommended.

Additional reported applications (for the relevant formats) include: Blocking of CTLA-4/B7-1 interaction and blocking of CTLA-4-mediated inhibitory function to promote T cell expansion1,2.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of frozen or paraffin-embedded tissue sections (IHC-F or IHC-P).

Application References (PubMed link indicates BioLegend citation)

May K, et al. 2005. Blood 105:1114. (Block)
Lute K, et al. 2005. Blood 106:3127. (Block)

RRID

AB_2566296 (BioLegend Cat. No. 349923) AB_2566297 (BioLegend Cat. No. 349924) AB_2566298 (BioLegend Cat. No. 349925) AB_2566083 (BioLegend Cat. No. 349915) AB_2566084 (BioLegend Cat. No. 349916) AB_2566085 (BioLegend Cat. No. 349917)

Antigen Details

Structure: Ig superfamily, 33 kD
Distribution: Activated T cells and B cells
Function: Negative regulator of T cell activation
Interaction: Antigen presenting cells, such as dendritic cells
Ligand/Receptor: B7-1 (CD80), B7-2 (CD86)
Cell Type: B cells, T cells, Tregs
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay N, et al. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.
2. Kuiper H, et al. 1995. J. Immunol. 155:1776.
3. Lindsten T, et al. 1993. J. Immunol. 151:3489.
4. Morton P, et al. 1996. J. Immunol. 156:1047.
Gene ID: 1493 View all products for this Gene ID
UniProt: View information about CD152 on UniProt.org

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