Alexa Fluor® 647 anti-human CD152 (CTLA-4) Ant-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1488	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Extracellular domain of human CTLA-4 and human IgG1 Fc fusion protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

ELISA Detection: The biotinylated L3D10 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified A3.6B10.G1 antibody (Cat. No. 525401) as the capture antibody and recombinant human CTLA-4 (Cat. No. 591909) as the standard.

Flow Cytometry: The fluorochrome-labeled L3D10 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify CTLA-4-producing cells within mixed cell populations.

Note: For testing human soluble CTLA-4 in serum, plasma or cell culture supernatant, LEGEND MAX? Human Soluble CTLA-4 ELISA Kit with Pre-coated Plates (Cat. No. 437407) are specially developed and recommended.

Additional reported applications (for the relevant formats) include: Blocking of CTLA-4/B7-1 interaction and blocking of CTLA-4-mediated inhibitory function to promote T cell expansion¹^,².

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of frozen or paraffin-embedded tissue sections (IHC-F or IHC-P).

Application References (PubMed link indicates BioLegend citation)

May K, et al. 2005. Blood 105:1114. (Block)
Lute K, et al. 2005. Blood 106:3127. (Block)

Product Citations

Obradovic A, et al. 2021. Cell. 184(11):2988-3005.e16. PubMed
Chen W, et al. 2021. MAbs. 13:1914359. PubMed

RRID

AB_2566184 (BioLegend Cat. No. 349919) AB_2566185 (BioLegend Cat. No. 349920)

Antigen Details

Structure: Ig superfamily, 33 kD
Distribution: Activated T cells and B cells
Function: Negative regulator of T cell activation
Interaction: Antigen presenting cells, such as dendritic cells
Ligand/Receptor: B7-1 (CD80), B7-2 (CD86)
Cell Type: B cells, T cells, Tregs
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay N, et al. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.
2. Kuiper H, et al. 1995. J. Immunol. 155:1776.
3. Lindsten T, et al. 1993. J. Immunol. 151:3489.
4. Morton P, et al. 1996. J. Immunol. 156:1047.
Gene ID: 1493 View all products for this Gene ID
UniProt: View information about CD152 on UniProt.org

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