Ultra-LEAF™ Purified anti-mouse α-GalC
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse α-GalC
- 产品类别：
- 抗体
- 产品编号：
- 140517
- 产品应用：
- 140517
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- α-GalCer:mCD1d1 complexes coupled to Mycobacterium tuberculosis PPD
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block, ICC?- Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: Surface and intracellular immunofluorescence2 and block a-GalCer:mCD1d1 complex induced iNKT activation1.
- Application References
(PubMed link indicates BioLegend citation) -
- Yu KO, et al. 2007. J. Immunol. Methods 323:11. (Block)
- Im JS, et al. 2009. Immunity 30:888. (IF)
- RRID
- AB_2814042 (BioLegend Cat. No. 140517) AB_2814043 (BioLegend Cat. No. 140518)
- Distribution
-
NKT cells
- Cell Type
- B cells, Dendritic cells, NKT cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, MHC Antigens, TCRs
- Antigen References
-
1. Arrenberg P, et al. 2010. P. Natl. Acad. Sci. USA 107:10984.
2. Mattarollo SR, et al. 2010. J. Immunol. 184:5663.
3. Wang J, et al. 2010. P. Natl. Acad. Sci. USA 107:1535.
4. Nieuwenhuis EE, et al. 2009. J. Clin. Invest. 119:1241.
5. Sullivan BA and Kronenberg M. 2005. J. Clin. Invest. 115:2328.
- Gene ID
- 12479 View all products for this Gene ID
- UniProt
- View information about alpha-GalCer:CD1d complex on UniProt.org
