Biotin anti-mouse α-GalCer:CD1d complex Anti
- 产品名称:
- Biotin anti-mouse α-GalCer:CD1d complex Anti
- 产品类别:
- 抗体
- 产品编号:
- 140510
- 产品应用:
- 140510
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- α-GalCer:mCD1d1 complexes coupled to Mycobacterium tuberculosis PPD
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC -?Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: Surface and intracellular immunofluorescence2 and block a-GalCer:mCD1d1 complex induced iNKT activation1.
- Application References
(PubMed link indicates BioLegend citation) -
- Yu KO, et al. 2007. J. Immunol. Methods 323:11. (Block)
- Im JS, et al. 2009. Immunity 30:888. (IF)
- RRID
- AB_2749919 (BioLegend Cat. No. 140510)
- Distribution
-
NKT cells
- Cell Type
- B cells, Dendritic cells, NKT cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, MHC Antigens, TCRs
- Antigen References
-
1. Arrenberg P, et al. 2010. P. Natl. Acad. Sci. USA 107:10984.
2. Mattarollo SR, et al. 2010. J. Immunol. 184:5663.
3. Wang J, et al. 2010. P. Natl. Acad. Sci. USA 107:1535.
4. Nieuwenhuis EE, et al. 2009. J. Clin. Invest. 119:1241.
5. Sullivan BA and Kronenberg M. 2005. J. Clin. Invest. 115:2328.
- Gene ID
- 12479 View all products for this Gene ID
- UniProt
- View information about alpha-GalCer:CD1d complex on UniProt.org