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首页 > 抗体蛋白 > 抗体
PE anti-human HLA-DR Antibody
产品名称：
PE anti-human HLA-DR Antibody
产品类别：
抗体
产品编号：
307605
产品应用：
307605
[价格]
规格 价格 库存
25tests ￥ 864 1

产品详情

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Chimpanzee, Dog, Common Marmoset, Squirrel Monkey, Cotton-topped Tamarin
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the a? heterodimer.19

Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8, in vitro blocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7,?immunohistochemical staining of acetone-fixed frozen sections4,5, and spatial biology (IBEX)21,22. For sensitive functional assays, we recommend using the Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) (Cat. No. 307648, 307665 - 307669).

Application References

(PubMed link indicates BioLegend citation)
  1. Brodsky F. 1984. Immunogenetics 19:179.
  2. Robbins P, et al. 1987. Human Immunol. 18:301.
  3. Stites D, et al. 1986. Clin. Immunol. Immunopathol. 38:161.
  4. Warnke R, et al. 1980. J. Histochem. Cytochem. 28:771. (IHC)
  5. Engleman E, et al. 1981. P. Natl. Acad. Sci. USA 78:1791. (IHC)
  6. Zipf T, et al. 1981. Cancer Res. 41:4786.
  7. Goodier M, et al. 2000. J. Immunol. 165:139. (Depletion)
  8. Esser M, et al. 2001. J. Virol. 75:6173. (IP, WB)
  9. Kalka-Moll WM, et al. 2002. J. Immunol. 169:6149. (Block)
  10. Wang RF, et al. 1999. Science 284:1351. (Block)
  11. Zaba LC, et al. 2007. J. Exp. Med. 204:3183. PubMed
  12. Fujita H, et al. 2009. P. Natl. Acad. Sci. USA 106:21795. PubMed
  13. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  14. Goncalves RM, et al. 2010. Infect. Immun. 78:4763. PubMed
  15. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  16. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  17. Stein R, et al. 2011. Leuk. Lymphoma 52:273.
  18. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
  19. Moro M, et al. 2005. BMC Immunol. 6:24.
  20. Lauterbach N, et al. 2014. Mol Immunol. 59:19. PubMed
  21. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  22. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Sonoda S, et al. 2022. STAR Protoc. 3:101386. PubMed
  2. Bettencourt P, et al. 2020. NPJ Vaccines. 5:2. PubMed
  3. Liu C, et al. 2022. Regen Ther. 21:192. PubMed
  4. Xin Y, et al. 2021. Stem Cell Res Ther. 49:12. PubMed
  5. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  6. Wang F, et al. 2018. Oncogenesis. 7:41. PubMed
  7. Guney-Esken G, et al. 2021. Stem Cell Res Ther. 12:287. PubMed
  8. Karim F, et al. 2021. Elife. 10:. PubMed
  9. Thwe PM et al. 2017. Cell metabolism. 26(3):558-567 . PubMed
  10. Serra–Peinado C, et al. 2019. Nat Commun. 10:3705. PubMed
  11. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  12. Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
  13. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  14. Sugawara E, et al. 2020. Autophagy. 1.323611111. PubMed
  15. Asowata OE, et al. 2021. JCI Insight. 6:. PubMed
  16. Bellini N, et al. 2022. iScience. 25:105234. PubMed
  17. Fletcher H, et al. 2016. Nat Commun. 7: 11290. PubMed
  18. Gieseke F, et al. 2010. Blood. 116:3770. PubMed
  19. Blank A, et al. 2021. J Pathol. 253:160. PubMed
  20. Kan S, et al. 2020. Int J Oncol. 57:1047. PubMed
  21. Wang J, et al. 2020. Cell. 183:1264. PubMed
  22. Iwabuchi R, et al. 2021. Frontiers in Immunology. 12:643040. PubMed
  23. Bettencourt P, et al. 2020. NPJ Vaccines. 5:02. PubMed
  24. Zhang J, et al. 2022. Int J Mol Sci. 23:. PubMed
  25. Jost M, et al. 2021. eLife. 10:00. PubMed
  26. Malekghasemi S, et al. 2020. Cell Biol Int. 44:2031. PubMed
  27. Yu C, et al. 2020. Sci Rep. 10:14521. PubMed
  28. Zangeneh Z, et al. 2022. Iran J Basic Med Sci. 25:474. PubMed
  29. Wang X, et al. 2021. Front Endocrinol (Lausanne). 12:666195. PubMed
  30. Schupp J, et al. 2021. Int J Mol Sci. 22:00. PubMed
  31. Liakopoulos V, et al. 2018. Sci Rep. 8:13964. PubMed
  32. Fraccarollo D, et al. 2021. eLife. 0.416666666666667. PubMed
  33. Reichwald K, et al. 2014. PLoS One. 9:85793. PubMed
  34. Crane A, et al. 2018. Alzheimers Dement. 14:243. PubMed
  35. Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed
  36. Fredriksson K, et al. 2014. J Immunol. 192:4497. PubMed
  37. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  38. Zhang Q, et al. 2021. Bone Joint Res. 10:226. PubMed
  39. Bhat K, et al. 2010. J Biol Chem. 285:25893. PubMed
  40. Hakki S, et al. 2022. Sci Rep. 12:1427. PubMed
  41. Thirimanne HN, et al. 2022. Elife. 11:. PubMed
  42. Labedz-Maslowska A, et al. 2020. Int J Mol Sci. 21:. PubMed
  43. Rinaldi S, et al. 2021. PLoS Pathog. 17:e1009533. PubMed
  44. Zarobkiewicz M, et al. 2020. Cancers (Basel). 12:00. PubMed
  45. Ramaseri Sunder S, et al. 2020. J Interferon Cytokine Res. 40:310. PubMed
  46. Morgan J, et al. 2015. Biosci Rep. 35: 00233. PubMed
  47. Wang B, et al. 2018. Mol Ther Nucleic Acids. 0.548611111. PubMed
  48. Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
  49. Pan C, et al. 2019. Int J Mol Med. 44:1629. PubMed
  50. Gorby C, et al. 2020. Sci Signal. :13. PubMed
  51. Upasani V, et al. 2019. Front Immunol. 2.152777778. PubMed
  52. Li B, et al. 2019. Oncogenesis. 8:17. PubMed
  53. Wei X, et al. 2019. Int J Mol Med. 44:1425. PubMed
  54. Wang G, et al. 2018. Int J Mol Med. 41:791. PubMed
RRID
AB_314683 (BioLegend Cat. No. 307605) AB_314684 (BioLegend Cat. No. 307606)

Antigen Details

Structure
Ig superfamily, MHC class II, heterodimeric transmembrane protein, 36 kD heavy and 27 kD light chain
Distribution

B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs

Function
Peptide presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177.
2. Terstappen L, et al. 1990. J. Leukocyte Biol. 48:138.
3. Edwards JA, et al. 1986. J. Immunol. 137:490.
4. van Es A, et al. 1984. Transplantation 37:65.
5. O'Doherty U, et al. 1994. Immunology 82:487.
6. Thomas R, et al. 1994. J. Immunol. 153:4016.
7. Grouard G, et al. 1996. Nature 384:364.

Gene ID
3122 View all products for this Gene ID 3123 View all products for this Gene ID
UniProt
View information about HLA-DR on UniProt.org

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